STAT4 is a target of the hematopoietic zinc-finger transcription factor Ikaros in T cells

STAT4 is a target of the hematopoietic zinc-finger transcription factor Ikaros in T cells

STAT4 is a transcription factor activated in response to IL-12, and is involved in Th1 cell development. The molecular mechanisms controlling the transcription of the STAT4 gene are however, unclear. Sequence comparison of the 5′ flanking regions of human, mouse and pufferfish (Fugu rubripes) Stat4...

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Veröffentlicht in:FEBS letters 2005-08, Vol.579 (20), p.4470-4478
Hauptverfasser: Yap, Wai-Ho, Yeoh, Elizabeth, Tay, Alice, Brenner, Sydney, Venkatesh, Byrappa
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Base Sequence
Binding Sites
CNS
Comparative genomics
conserved non-coding sequence
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
electrophoretic mobility shift assay
EMSA
Fugu rubripes
Gene regulation
Genome
Hematopoiesis - genetics
Humans
Ikaros
Ikaros Transcription Factor
Jurkat Cells
lipopolysaccharide
LPS
Mice
Molecular Sequence Data
Promoter Regions, Genetic
RACE
rapid amplification of cDNA ends
reverse transcription
RNA Interference
signal transducer and activator of transcription
siRNA
small interfering RNA
STAT
STAT4 Transcription Factor
T-Lymphocytes - metabolism
Takifugu - genetics
TFBS
Trans-Activators - genetics
transcription factor binding site
Transcription factors
Transcription Factors - metabolism
transcription start site
Transcription, Genetic
Transcriptional Activation - genetics
TSS
untranslated region
UTR
Zinc Fingers
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Beschreibung
Zusammenfassung:STAT4 is a transcription factor activated in response to IL-12, and is involved in Th1 cell development. The molecular mechanisms controlling the transcription of the STAT4 gene are however, unclear. Sequence comparison of the 5′ flanking regions of human, mouse and pufferfish (Fugu rubripes) Stat4 genes revealed a high frequency of Ikaros (Ik) binding elements in all three species. We then investigated the role of Ik binding elements in the human STAT4 promoter using Jurkat T cells. Transactivation, electrophoretic mobility shift assay and RNA interference-mediated gene knockdown experiments revealed that Ik is involved in the regulation of STAT4 in human T cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2005.07.018