Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants

To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell e...

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Veröffentlicht in:	Biotechnology letters 2007-12, Vol.29 (12), p.2007-2012
Hauptverfasser:	Bai, Jie-Ying, Zeng, Lin, Hu, Yuan-Lei, Li, Yan-Fang, Lin, Zhong-Ping, Shang, Shi-Chen, Shi, Yan-Sheng
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals bioactive properties Biological and medical sciences Biotechnology Blotting, Northern Cell Proliferation - drug effects Chi-Square Distribution Chlorocebus aethiops Codon Epidermal Growth Factor - metabolism Epidermal Growth Factor - pharmacology Fundamental and applied biological sciences. Psychology Genetic Vectors Human epidermal growth factor Humans Matrix attachment regions Mitogens - pharmacology Nicotiana - genetics Optimized codons Plants, Genetically Modified Proteins Recombinant Proteins - metabolism Tobacco Vero Cells
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Zusammenfassung:	To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/s10529-007-9438-y