Type IV collagen degradation in the myocardial basement membrane after unloading of the failing heart by a left ventricular assist device

After left ventricular assist device (LVAD) support in patients with end-stage cardiomyopathy, cardiomyocytes decrease in size. We hypothesized that during this process, known as reverse remodeling, the basement membrane (BM), which is closely connected to, and forms the interface between the cardio...

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Veröffentlicht in:	Laboratory investigation 2007-11, Vol.87 (11), p.1125-1137
Hauptverfasser:	Bruggink, Annette H, van Oosterhout, Matthijs F M, de Jonge, Nicolaas, Cleutjens, Jack P M, van Wichen, Dick F, van Kuik, Joyce, Tilanus, Marcel G J, Gmelig-Meyling, Frits H J, van den Tweel, Jan G, de Weger, Roel A
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult basement membrane Basement Membrane - metabolism Basement Membrane - pathology Biological and medical sciences Biotechnology Collagen Type IV - metabolism Endothelial Cells - metabolism Female Fundamental and applied biological sciences. Psychology Heart Failure - metabolism Heart Failure - pathology Heart Failure - therapy Heart-Assist Devices - adverse effects Humans Immunohistochemistry In Situ Hybridization Investigative techniques, diagnostic techniques (general aspects) LVAD support Macrophages - metabolism Male Matrix Metalloproteinase 2 - metabolism Medical sciences Middle Aged MMP-2 Myocytes, Cardiac - metabolism Myocytes, Cardiac - pathology reverse remodeling Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism T-Lymphocytes - metabolism type IV collagen Ventricular Dysfunction, Left - metabolism Ventricular Dysfunction, Left - pathology Ventricular Dysfunction, Left - therapy
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Zusammenfassung:	After left ventricular assist device (LVAD) support in patients with end-stage cardiomyopathy, cardiomyocytes decrease in size. We hypothesized that during this process, known as reverse remodeling, the basement membrane (BM), which is closely connected to, and forms the interface between the cardiomyocytes and the extracellular matrix, will be severely affected. Therefore, the changes in the myocardial BM in patients with end-stage heart failure before and after LVAD support were studied. The role of MMP-2 in this process was also investigated. Transmission electron microscopy showed that the BM thickness decreased post-LVAD compared to pre-LVAD. Immunohistochemistry indicated a reduced immunoreactivity for type IV collagen in the BM after LVAD support. Quantitative PCR showed a similar mRNA expression for type IV collagen pre- and post-LVAD. MMP-2 mRNA almost doubled post-LVAD (P
ISSN:	0023-6837 1530-0307
DOI:	10.1038/labinvest.3700670