DNA Mutation Detection Using Denaturing High‐Performance Liquid Chromatography (DHPLC)

DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single‐base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species un...

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Veröffentlicht in:Current Protocols in Human Genetics 2006-01, Vol.48 (1), p.7.10.1-7.10.14
Hauptverfasser: Yu, Bing, Sawyer, Nicole A., Chiu, Christine, Oefner, Peter J., Underhill, Peter A.
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Sprache:eng
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Zusammenfassung:DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single‐base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high‐throughput mutation detection and SNP discovery.
ISSN:1934-8266
1934-8258
DOI:10.1002/0471142905.hg0710s48