Screening for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala koidz

The aim of this study was to screen for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala Koidz. The rhizomes of Atractylodes macrocephala were treated with supercritical CO 2 fluid and the extract was separated by normal-phase and reverse-phase column chromato...

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Veröffentlicht in:Journal of ethnopharmacology 2007-11, Vol.114 (2), p.212-217
Hauptverfasser: Li, Cui-Qin, He, Lang-Chong, Dong, Hai-Yan, Jin, Ju-Qing
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Sprache:eng
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Zusammenfassung:The aim of this study was to screen for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala Koidz. The rhizomes of Atractylodes macrocephala were treated with supercritical CO 2 fluid and the extract was separated by normal-phase and reverse-phase column chromatography. The separated samples were screened with white blood cell membrane (WBCM) chromatography (WBCM-C). The anti-inflammatory effects of these fractions and components were tested pharmacologically in vivo. The results indicated that the retention characteristics of the petrol-ether (1:1, v/v) fraction (BZC-2) of the supercritical CO 2 extract, the atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2 E,8 E,10 E-trien-4, 6-diyn-1-ol isolated from BZC-2 as active fractions and components were similar to that of dexamethasone in WBCM-C. Therefore, they may act on WBCM and its receptors. BZC-2 has shown anti-inflammatory effects in acute and chronic inflammation models in rats and mice. Oral administration of atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2 E,8 E,10 E-trien-4,6-diyn-1-ol produced significant anti-inflammatory effects in acute and chronic inflammation models in mice. The screening results with WBCM-C were correlated significantly with pharmacological effects in vivo. Atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2 E,8 E,10 E-trien-4,6-diyn-1-ol were the main components of Atractylodes macrocephala that were effective as anti-inflammatory agents.
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2007.08.002