Production of xylitol by metabolically engineered strains of Bacillus subtilis

Xylitol-phosphate dehydrogenase (XPDH) genes from several Gram-positive bacteria were isolated and expressed in Bacillus subtilis. The substrate specificities of the recombinant XPDH enzymes were compared and it was found that the XPDH enzymes of Lactobacillus rhamnosus and Clostridium difficile had...

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Veröffentlicht in:	Journal of biotechnology 2007-01, Vol.128 (1), p.24-31
Hauptverfasser:	Povelainen, Mira, Miasnikov, Andrei N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus subtilis Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacillus subtilis - metabolism Bacterial Proteins - metabolism Biological and medical sciences Biotechnology Clostridium difficile Clostridium difficile - enzymology Clostridium difficile - genetics Fermentation Fundamental and applied biological sciences. Psychology Genetic Engineering Glucose - metabolism Lactobacillus rhamnosus Lactobacillus rhamnosus - enzymology Lactobacillus rhamnosus - genetics Recombinant Proteins - metabolism Ribulosephosphates - metabolism Sugar Alcohol Dehydrogenases - genetics Xylitol Xylitol - metabolism Xylitol-phosphate dehydrogenase Xylose - metabolism
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Zusammenfassung:	Xylitol-phosphate dehydrogenase (XPDH) genes from several Gram-positive bacteria were isolated and expressed in Bacillus subtilis. The substrate specificities of the recombinant XPDH enzymes were compared and it was found that the XPDH enzymes of Lactobacillus rhamnosus and Clostridium difficile had the highest selectivity towards d-xylulose 5-phosphate. Expression of these two XPDH enzymes in d-ribulose and d-xylulose producing B. subtilis strain resulted in strains of B. subtilis capable of converting d-glucose into xylitol at around 23% yield.
ISSN:	0168-1656 1873-4863
DOI:	10.1016/j.jbiotec.2006.09.008