Spectral study of the interaction of DNA with benzothiazolyl-benz-alpha-chromene

Absorption and luminescence excitation and emission spectra of newly synthesized 2-(4-methylphenylimino)-3-(2 -benzothiazolyl)benz-alpha-chromene (BCBT) have been studied in the presence of various DNA concentrations. BCBT is characterized by the existence of two different fluorescent systems, exhib...

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Veröffentlicht in:	Biochemistry (Moscow) 2007-08, Vol.72 (8), p.901-909
Hauptverfasser:	Sibirtsev, V S, Garabadzhiu, A V
Format:	Artikel
Sprache:	eng
Schlagworte:	Benzopyrans - chemical synthesis Benzopyrans - chemistry Benzothiazoles - chemical synthesis Benzothiazoles - chemistry DNA - chemistry Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Intercalating Agents - chemical synthesis Intercalating Agents - chemistry
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Zusammenfassung:	Absorption and luminescence excitation and emission spectra of newly synthesized 2-(4-methylphenylimino)-3-(2 -benzothiazolyl)benz-alpha-chromene (BCBT) have been studied in the presence of various DNA concentrations. BCBT is characterized by the existence of two different fluorescent systems, exhibiting radiationless fluorescence resonance energy transfer between them. In the range of molar ratios of polynucleotide/dye concentrations from 0 to 50, BCBT preferentially intercalates into DNA due to its benz-alpha-chromene fragment, whereas the 2-benzothiazolyl fragment is responsible for fluorescence.
ISSN:	0006-2979 1608-3040 0320-9725
DOI:	10.1134/S0006297907080123