Complex patterns of the HIV-1 epidemic in Kuala Lumpur, Malaysia: Evidence for expansion of circulating recombinant form CRF33_01B and detection of multiple other recombinants

Abstract The HIV protease-reverse transcriptase (PR-RT) (1047 bp), gp120-env (891 bp) and gp41-env (547 bp) regions from the plasma of 115 HIV-1-infected patients in Kuala Lumpur (KL), Malaysia were sequenced. Detailed phylogenetic and bootscanning analyses were performed to determine the mosaic str...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 2007-10, Vol.367 (2), p.288-297
Hauptverfasser: Wang, Bin, Lau, Katherine A, Ong, Lai-Yee, Shah, Meet, Steain, Megan C, Foley, Brian, Dwyer, Dominic E, Chew, Choo Beng, Kamarulzaman, Adeeba, Ng, Kee Peng, Saksena, Nitin K
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Sprache:eng
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Zusammenfassung:Abstract The HIV protease-reverse transcriptase (PR-RT) (1047 bp), gp120-env (891 bp) and gp41-env (547 bp) regions from the plasma of 115 HIV-1-infected patients in Kuala Lumpur (KL), Malaysia were sequenced. Detailed phylogenetic and bootscanning analyses were performed to determine the mosaic structure of the HIV-1 strains and their recombination breakpoint(s). Among the 50 patient samples in which all three regions could be amplified, the HIV-1 CRF01_AE subtype (46%) was predominant followed by subtypes B (10%) and B′ (6%). A total of 9/50 (18%) patients were infected with a CRF01_AE/B inter-subtype recombinant, displaying a recombinant form (RF)PR-RT , CRF01_AE gp120-env and CRF01_AE gp41-env . This RF was derived from the Thai variants of CRF01_AE and B′ subtype, with two distinct B′ subtype segments in the backbone of CRF01_AE, similar to the newly identified CRF33_01B. In addition, one sample demonstrated a close structural relationship with the new CRF33_01B in the PR-RT region but displayed B′ segment in part of the env region (RFPR-RT , CRF01_AE/B′ gp120-env and B′ gp41-env ) indicating continuing evolution of CRF33_01B. The remaining 18% of samples were identified as unique recombinant forms (URFs).
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2007.05.033