Component and Antioxidant Properties of Polysaccharide Fractions Isolated from Angelica sinensis (OLIV.) DIELS

An analytical method of high performance capillary electrophoresis (HPCE) was developed to simultaneously separate and identify the component monosaccharides of Angelica sinensis polysaccharide fractions (APFs), named APF1, APF2 and APF3. The predominant sugars in APFs were identified as arabinose,...

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Veröffentlicht in:	Biological & Pharmaceutical Bulletin 2007/10/01, Vol.30(10), pp.1884-1890
Hauptverfasser:	Yang, Xingbin, Zhao, Yan, Zhou, Yanjuan, Lv, You, Mao, Jieli, Zhao, Ping
Format:	Artikel
Sprache:	eng
Schlagworte:	Angelica sinensis Angelica sinensis - chemistry Animals antioxidant activity Antioxidants - isolation & purification Antioxidants - pharmacology Capillary Electrochromatography capillary electrophoresis Carbohydrates - analysis Cells, Cultured Chromatography, Gel Hydrogen Peroxide - chemistry Indicators and Reagents L-Lactate Dehydrogenase - metabolism macrophage Macrophages, Peritoneal - drug effects Macrophages, Peritoneal - metabolism Mice Mice, Inbred BALB C Nitrites - chemistry Oxidative Stress - drug effects Plant Roots - chemistry polysaccharide fraction Polysaccharides - isolation & purification Polysaccharides - pharmacology Reactive Oxygen Species - metabolism Superoxide Dismutase - metabolism
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Zusammenfassung:	An analytical method of high performance capillary electrophoresis (HPCE) was developed to simultaneously separate and identify the component monosaccharides of Angelica sinensis polysaccharide fractions (APFs), named APF1, APF2 and APF3. The predominant sugars in APFs were identified as arabinose, glucose, rhamnose, galactose and galacturonic acid as well as trace amount of mannose and glucuronic acid, and the fractionation altered significantly the distribution of component monosaccharides in APFs. APF3 was the most active fraction to effectively inhibit H2O2-caused decrease of cell viability, lactate dehydrogenase (LDH) leakage and malondialdehyde (MDA) formation, and also reduced H2O2-caused decline of superoxide dismutase (SOD) activity and glutathione (GSH) depletion (p
ISSN:	0918-6158 1347-5215
DOI:	10.1248/bpb.30.1884