Homogeneous chemiluminescent assays for free choline in human plasma and whole blood
Choline was oxidized in the presence of choline oxidase and the hydrogen peroxide generated was detected using a chemiluminescent acridinium-9-carboxamide. The dose response for choline (0–150 μM) was established in buffer and was validated for the quantification of choline in human plasma and whole...
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| Veröffentlicht in: | Analytica chimica acta 2006-10, Vol.579 (1), p.61-67 |
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| container_title | Analytica chimica acta |
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| creator | Adamczyk, Maciej Brashear, R. Jeffrey Mattingly, Phillip G. Tsatsos, Panagiota H. |
| description | Choline was oxidized in the presence of choline oxidase and the hydrogen peroxide generated was detected using a chemiluminescent acridinium-9-carboxamide. The dose response for choline (0–150
μM) was established in buffer and was validated for the quantification of choline in human plasma and whole blood. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (4
μL) and short analysis time (
0.98, plasma;
R
>
0.97, whole blood) with LC-MS/MS. |
| doi_str_mv | 10.1016/j.aca.2006.07.020 |
| format | Article |
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μM) was established in buffer and was validated for the quantification of choline in human plasma and whole blood. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (4
μL) and short analysis time (<5
s per well). The new assay(s) correlated well (
R
>
0.98, plasma;
R
>
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μM) was established in buffer and was validated for the quantification of choline in human plasma and whole blood. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (4
μL) and short analysis time (<5
s per well). The new assay(s) correlated well (
R
>
0.98, plasma;
R
>
0.97, whole blood) with LC-MS/MS.</description><subject>Analytical chemistry</subject><subject>Chemical and thermal methods</subject><subject>Chemiluminescence</subject><subject>Chemistry</subject><subject>Choline analysis</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Exact sciences and technology</subject><subject>Liquid chromatography-electrospray ionization tandem mass spectrometry correlation</subject><subject>Other chromatographic methods</subject><subject>Plasma</subject><subject>Spectrometric and optical methods</subject><subject>Whole blood</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkMFu2zAMhoVhxZq1fYBeCl3Wm11SsiMbOw3Bug4osEt6FmSJXhXYVibFG_L2VZYAvbUngvg_EuTH2DVCiYDLu01prCkFwLIEVYKAD2yBjZJFJUX1kS0AQBZiqeCcfU5pk1uBUH1i56iUkEo0C7Z-CGP4TROFOXH7TKMf5tFPlCxNO25SMvvE-xB5H4kyEIYccj_x53k0E98OJo2Gm8nxfzkj3g0huEt21psh0dWpXrCn--_r1UPx-OvHz9W3x8JWKHZF37uqJVO5GkTbIqJ0tu5EQ0I0EkgSSQdW2U6RzUGnmtrViA6dUlK1Sl6w2-PebQx_Zko7Pfp8-DCY___oZSMz38p3QdHWdY3qAOIRtDGkFKnX2-hHE_caQR-c643OzvXBuQals_M8c3NaPncjudeJk-QMfDkBJlkz9NFM1qdXrsGqBagz9_XIUXb211PUyXqaLDkfye60C_6NM14AA1meng</recordid><startdate>20061002</startdate><enddate>20061002</enddate><creator>Adamczyk, Maciej</creator><creator>Brashear, R. Jeffrey</creator><creator>Mattingly, Phillip G.</creator><creator>Tsatsos, Panagiota H.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20061002</creationdate><title>Homogeneous chemiluminescent assays for free choline in human plasma and whole blood</title><author>Adamczyk, Maciej ; Brashear, R. Jeffrey ; Mattingly, Phillip G. ; Tsatsos, Panagiota H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-ffd49ea4d502991113dc5b28e22830e3ee3d0c7cb7ecc5bb785d511d1d7737973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analytical chemistry</topic><topic>Chemical and thermal methods</topic><topic>Chemiluminescence</topic><topic>Chemistry</topic><topic>Choline analysis</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Exact sciences and technology</topic><topic>Liquid chromatography-electrospray ionization tandem mass spectrometry correlation</topic><topic>Other chromatographic methods</topic><topic>Plasma</topic><topic>Spectrometric and optical methods</topic><topic>Whole blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adamczyk, Maciej</creatorcontrib><creatorcontrib>Brashear, R. Jeffrey</creatorcontrib><creatorcontrib>Mattingly, Phillip G.</creatorcontrib><creatorcontrib>Tsatsos, Panagiota H.</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adamczyk, Maciej</au><au>Brashear, R. Jeffrey</au><au>Mattingly, Phillip G.</au><au>Tsatsos, Panagiota H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Homogeneous chemiluminescent assays for free choline in human plasma and whole blood</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2006-10-02</date><risdate>2006</risdate><volume>579</volume><issue>1</issue><spage>61</spage><epage>67</epage><pages>61-67</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>Choline was oxidized in the presence of choline oxidase and the hydrogen peroxide generated was detected using a chemiluminescent acridinium-9-carboxamide. The dose response for choline (0–150
μM) was established in buffer and was validated for the quantification of choline in human plasma and whole blood. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (4
μL) and short analysis time (<5
s per well). The new assay(s) correlated well (
R
>
0.98, plasma;
R
>
0.97, whole blood) with LC-MS/MS.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17723728</pmid><doi>10.1016/j.aca.2006.07.020</doi><tpages>7</tpages></addata></record> |
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| ispartof | Analytica chimica acta, 2006-10, Vol.579 (1), p.61-67 |
| issn | 0003-2670 1873-4324 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Analytical chemistry Chemical and thermal methods Chemiluminescence Chemistry Choline analysis Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Liquid chromatography-electrospray ionization tandem mass spectrometry correlation Other chromatographic methods Plasma Spectrometric and optical methods Whole blood |
| title | Homogeneous chemiluminescent assays for free choline in human plasma and whole blood |
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