Homogeneous chemiluminescent assays for free choline in human plasma and whole blood

Choline was oxidized in the presence of choline oxidase and the hydrogen peroxide generated was detected using a chemiluminescent acridinium-9-carboxamide. The dose response for choline (0–150 μM) was established in buffer and was validated for the quantification of choline in human plasma and whole...

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Veröffentlicht in:	Analytica chimica acta 2006-10, Vol.579 (1), p.61-67
Hauptverfasser:	Adamczyk, Maciej, Brashear, R. Jeffrey, Mattingly, Phillip G., Tsatsos, Panagiota H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Chemical and thermal methods Chemiluminescence Chemistry Choline analysis Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Liquid chromatography-electrospray ionization tandem mass spectrometry correlation Other chromatographic methods Plasma Spectrometric and optical methods Whole blood
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Beschreibung
Zusammenfassung:	Choline was oxidized in the presence of choline oxidase and the hydrogen peroxide generated was detected using a chemiluminescent acridinium-9-carboxamide. The dose response for choline (0–150 μM) was established in buffer and was validated for the quantification of choline in human plasma and whole blood. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (4 μL) and short analysis time ( 0.98, plasma; R > 0.97, whole blood) with LC-MS/MS.
ISSN:	0003-2670 1873-4324
DOI:	10.1016/j.aca.2006.07.020