A cylinder-plate method for microbiological assay of clavulanic acid

Clavulanic acid is a natural occurring beta-lactam product of Streptomyces clavuligerus and is a potent inhibitor of bacterial beta-lactamases. The present work reports a microbiological assay based on the cylinder-plate method for determination of clavulanic acid. The assay is based on the inhibito...

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Veröffentlicht in:	Pharmeuropa scientific notes 2006-12, Vol.2006 (2), p.53-54
Hauptverfasser:	Hamedi, J, Shahverdi, A R, Samadi, N, Mohammadi, A, Shiran, M, Akhondi, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Agar Anti-Bacterial Agents - analysis Anti-Bacterial Agents - pharmacology Calibration Chromatography, High Pressure Liquid Clavulanic Acid - analysis Clavulanic Acid - pharmacology Escherichia coli - drug effects Escherichia coli - growth & development Microbial Sensitivity Tests Penicillin G - pharmacology Pharmaceutical Preparations - standards Quality Control Reproducibility of Results Technology, Pharmaceutical - methods
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Beschreibung
Zusammenfassung:	Clavulanic acid is a natural occurring beta-lactam product of Streptomyces clavuligerus and is a potent inhibitor of bacterial beta-lactamases. The present work reports a microbiological assay based on the cylinder-plate method for determination of clavulanic acid. The assay is based on the inhibitory effect of clavulanic acid in combination with penicillin G upon Escherichia coli ATCC 35218, which is used as the test organism. The correlation between clavulanic acid concentration and the inhibitory effect on E. coli was linear (r > 0.99) and in the range of 8-20 microg/ml. These results indicate that the proposed method is appropriate for the determination of clavulanic acid in commercial samples and can be used in routine quality control.
ISSN:	1814-2435