Fervidobacterium changbaicum sp. nov., a novel thermophilic anaerobic bacterium isolated from a hot spring of the Changbai Mountains, China

1 Key Laboratory for Molecular Enzymology and Engineering, Jilin University, Changchun 130023, PR China 2 School of Life Science, Northeast Normal University, Changchun 130024, PR China 3 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China Correspondence Yan Feng yfeng{at}mail.jlu.edu.cn A thermophilic, obligately anaerobic, rod-shaped bacterium (strain CBS-1 T ) was isolated from a hot spring mixture of water and mud of the Changbai Mountains, China. Strain CBS-1 T was found to be non-sporulating, Gram-negative, with optimal growth at 75–80 °C. It grew on a wide range of carbon sources, including glucose, lactose, maltose, starch, sorbitol and pyruvate amongst others. The DNA G+C content of strain CBS-1 T was 31.9 mol%. The 16S rRNA gene sequence analysis indicated that the strain was a member of the genus Fervidobacterium . The high concentration of C 16 : 0 (52.2 %) in the fatty acid profile of the cell envelope supported its inclusion as a member of the genus Fervidobacterium . On the basis of the low values of DNA–DNA hybridization (25.8 and 20.5 %) and phenotypic features, strain CBS-1 T represents a novel species of the genus Fervidobacterium , for which the name Fervidobacterium changbaicum sp. nov. is proposed. The type strain is CBS-1 T (=DSM 17883 T =JCM 13353 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CBS-1 T is AY878719 . An electron micrograph of cells of strain CBS-1 T , graphs showing the influence of temperature, pH and salt concentration on growth of cells of strain CBS-1 T and fatty acid compositions of strain CBS-1 T are available as supplementary material with the online version of this paper.