Enhanced concentration and isolation of Cyclospora cayetanensis oocysts from human fecal samples
Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from a...
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| Veröffentlicht in: | Journal of microbiological methods 2007-10, Vol.71 (1), p.75-77 |
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| container_end_page | 77 |
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| container_title | Journal of microbiological methods |
| container_volume | 71 |
| creator | Riner, Diana K. Mullin, Andrew S. Lucas, Sasha Y. Cross, John H. Lindquist, H.D. Alan |
| description | Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of
C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from adherent fecal debris and amenable to processing using flow cytometry. |
| doi_str_mv | 10.1016/j.mimet.2007.06.021 |
| format | Article |
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Psychology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Oocysts</subject><subject>Renocal-sucrose gradient</subject><subject>Sucrose</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoO4uLOjv0CQXPTWvfnoTtIHDzLsh7DgRc-xJh9shu7OmOoR5t-bcQb2pqei4KmX4n0Iec9ZyxlXt7t2SlNYWsGYbplqmeCvyIobLRoj--E1WVVKN5pxcU1uEHeM8V525g255loNRohhRX7ezc8wu-Cpy3XMS4El5ZnC7GnCPJ63HOnm6MaM-1yAOjiGBeYwY0KaszvigjSWPNHnwwQzjcHBSBGm_RjwLbmKMGJ4d5lr8uP-7vvmsXn69vB18-WpcZ2SS-PMoLnqowPwxoGKvY9MdVKA6oJnnRcOovBBbT2XvHcgtj2wPvDOK8a2g1yTT-fcfcm_DgEXOyV0YRzro_mAVhkpO230f0E-GD10FV8TeQZdyYglRLsvaYJytJzZkwG7s38N2JMBy5StBurVh0v8YTsF_3JzqbwCHy8AYO0pllp_whdu4LKvOZX7fOZCbe13CsWiS-GkKpXgFutz-ucjfwCidqd4</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Riner, Diana K.</creator><creator>Mullin, Andrew S.</creator><creator>Lucas, Sasha Y.</creator><creator>Cross, John H.</creator><creator>Lindquist, H.D. 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| fulltext | fulltext |
| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2007-10, Vol.71 (1), p.75-77 |
| issn | 0167-7012 1872-8359 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Centrifugation - methods Cyclospora - isolation & purification Cyclospora - physiology Cyclospora cayetanensis Cyclosporiasis - diagnosis Detachment solution Feces - parasitology Flow cytometry Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Humans Microbiology Mycology Oocysts Renocal-sucrose gradient Sucrose Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies |
| title | Enhanced concentration and isolation of Cyclospora cayetanensis oocysts from human fecal samples |
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