Enhanced concentration and isolation of Cyclospora cayetanensis oocysts from human fecal samples

Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from a...

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Veröffentlicht in:	Journal of microbiological methods 2007-10, Vol.71 (1), p.75-77
Hauptverfasser:	Riner, Diana K., Mullin, Andrew S., Lucas, Sasha Y., Cross, John H., Lindquist, H.D. Alan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Centrifugation - methods Cyclospora - isolation & purification Cyclospora - physiology Cyclospora cayetanensis Cyclosporiasis - diagnosis Detachment solution Feces - parasitology Flow cytometry Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Humans Microbiology Mycology Oocysts Renocal-sucrose gradient Sucrose Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Zusammenfassung:	Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from adherent fecal debris and amenable to processing using flow cytometry.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2007.06.021