High-throughput genotyping of filamentous fungus Aspergillus oryzae based on colony direct polymerase chain reaction

High-throughput genotyping of Aspergillus oryzae was achieved using an FTA card for the extraction of a genomic DNA template for polymerase chain reaction from a fungal colony growing on an agar plate. This method was then applied to detect other fungal species from agar slants and food materials. T...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2006-12, Vol.102 (6), p.572-574
Hauptverfasser:	Suzuki, Satoshi, Taketani, Hiroko, Kusumoto, Ken-Ichi, Kashiwagi, Yutaka
Format:	Artikel
Sprache:	eng
Schlagworte:	ASPERGILLUS ORYZAE Aspergillus oryzae - genetics Aspergillus oryzae - isolation & purification Biological and medical sciences Biotechnology Chromosome Mapping - methods colony polymerase chain reaction DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology GENE GENES GENOMAS GENOME Genome, Fungal - genetics GENOMES Genotype IDENTIFICACION IDENTIFICATION PCR Polymerase Chain Reaction - methods
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Beschreibung
Zusammenfassung:	High-throughput genotyping of Aspergillus oryzae was achieved using an FTA card for the extraction of a genomic DNA template for polymerase chain reaction from a fungal colony growing on an agar plate. This method was then applied to detect other fungal species from agar slants and food materials. This method offers a convenient tool for the genotyping of filamentous fungi without using an organic solvent or specialized equipment.
ISSN:	1389-1723 1347-4421
DOI:	10.1263/jbb.102.572