α-Glucosidase inhibitor proteins from Sesbania grandiflora flowers

Abstract Two alpha-glucosidase inhibitors were isolated from the flowers of SESBANIA GRANDIFLORA and named SGF60 and SGF90. The procedure involved extraction with phosphate buffer, precipitation with ammonium sulfate, ion-exchange chromatography on DEAE-cellulose and gel filtration on Superdex-200. These proteins were identified by using tandem mass spectrometry. The results show partial amino acid sequences of SGF60 similar to p27SJ, a protein from HYPERICUM PERFORATUM found to suppress HIV-1 gene expression. SGF90 matched a beta-glucosidase from ARABIDOPSIS THALIANA.