Characterization of CTX-M-22 and TEM-141 Encoded by a Single Plasmid from a Clinical Isolate of Enterobacter cloacae in China

We analyzed the resistance to expanded-spectrum cephalosporins of an Enterobacter cloacae clinical isolate, EC002, by transconjugation, isoelectric-focusing analysis, and cloning experiments. It produced two β-lactamases with isoelectric point values of 5.4 and 8.7, corresponding to TEM-141, a novel...

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Veröffentlicht in:Japanese Journal of Infectious Diseases 2007/09/27, Vol.60(5), pp.295-297
Hauptverfasser: Liu, Gang, Ling, Bao-Dong, Xie, Yong-En, Lin, Li, Zeng, Yun, Zhang, Xiang, Lei, Jun
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container_end_page 297
container_issue 5
container_start_page 295
container_title Japanese Journal of Infectious Diseases
container_volume 60
creator Liu, Gang
Ling, Bao-Dong
Xie, Yong-En
Lin, Li
Zeng, Yun
Zhang, Xiang
Lei, Jun
description We analyzed the resistance to expanded-spectrum cephalosporins of an Enterobacter cloacae clinical isolate, EC002, by transconjugation, isoelectric-focusing analysis, and cloning experiments. It produced two β-lactamases with isoelectric point values of 5.4 and 8.7, corresponding to TEM-141, a novel variant of TEM-1, and CTX-M-22, encoded by a transferable plasmid.
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source J-STAGE Free; MEDLINE; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals
subjects Base Sequence
beta-Lactam Resistance - genetics
beta-Lactamases - genetics
beta-Lactamases - metabolism
Cephalosporins - pharmacology
China
Drug Resistance, Bacterial - genetics
Enterobacter cloacae - enzymology
Enterobacter cloacae - genetics
Enterobacter cloacae - isolation & purification
Enterobacteriaceae Infections - drug therapy
Enterobacteriaceae Infections - microbiology
Humans
Klebsiella pneumoniae - genetics
Molecular Sequence Data
Plasmids - genetics
title Characterization of CTX-M-22 and TEM-141 Encoded by a Single Plasmid from a Clinical Isolate of Enterobacter cloacae in China
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