Visualization of Size-Selective Protein Separations on Spherical Mesoporous Silicates

Pores for effect: Spherical, mesoporous silica particles with pore sizes ranging from 28–127 Å and particle diameters of 5–10 μm were used to achieve size‐based separations of two proteins (see figure). This separation has been visualized using confocal scanning laser microscopy and confirmed with f...

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Veröffentlicht in:	Small (Weinheim an der Bergstrasse, Germany) Germany), 2006-05, Vol.2 (5), p.644-648
Hauptverfasser:	Katiyar, Amit, Pinto, Neville G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption calorimetry confocal laser microscopy Crystallization - methods Fluorescent Dyes Materials Testing Microscopy, Confocal - methods Microscopy, Fluorescence - methods Molecular Conformation Multiprotein Complexes - chemistry nanoporous materials Nanostructures - chemistry Nanostructures - ultrastructure Nanotechnology - methods Particle Size Porosity protein adsorption Protein Binding Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - ultrastructure Silicates - chemistry Silicon Dioxide - chemistry size selectivity Surface Properties
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Beschreibung
Zusammenfassung:	Pores for effect: Spherical, mesoporous silica particles with pore sizes ranging from 28–127 Å and particle diameters of 5–10 μm were used to achieve size‐based separations of two proteins (see figure). This separation has been visualized using confocal scanning laser microscopy and confirmed with flow microcalorimetry. The results show that mesoporous silicates can be developed as molecular‐sieve media for protein separation.
ISSN:	1613-6810 1613-6829
DOI:	10.1002/smll.200500473