One- and Two-Photon Excited Optical pH Probing for Cells Using Surface-Enhanced Raman and Hyper-Raman Nanosensors

We demonstrate spatially resolved probing and imaging of pH in live cells by mobile and biocompatible nanosensors using surface-enhanced Raman scattering (SERS) of 4-mercaptobenzoic acid (pMBA) on gold nanoaggregates. Moreover, we also show that this concept of pH nanosensors can be extended to two-...

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Veröffentlicht in:	Nano letters 2007-09, Vol.7 (9), p.2819-2823
Hauptverfasser:	Kneipp, Janina, Kneipp, Harald, Wittig, Burghardt, Kneipp, Katrin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biosensing Techniques - instrumentation Biosensing Techniques - methods Condensed matter: structure, mechanical and thermal properties Equipment Design Equipment Failure Analysis Exact sciences and technology General equipment and techniques Hydrogen-Ion Concentration Instruments, apparatus, components and techniques common to several branches of physics and astronomy Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties Mice Microscopy, Fluorescence, Multiphoton - instrumentation Microscopy, Fluorescence, Multiphoton - methods Nanotechnology - instrumentation Nanotechnology - methods NIH 3T3 Cells Physics Sensors (chemical, optical, electrical, movement, gas, etc.) remote sensing Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods Surfaces and interfaces thin films and whiskers (structure and nonelectronic properties)
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Zusammenfassung:	We demonstrate spatially resolved probing and imaging of pH in live cells by mobile and biocompatible nanosensors using surface-enhanced Raman scattering (SERS) of 4-mercaptobenzoic acid (pMBA) on gold nanoaggregates. Moreover, we also show that this concept of pH nanosensors can be extended to two-photon excitation by using surface-enhanced hyper-Raman scattering (SEHRS). In addition to the advantages of two-photon excitation, the SEHRS sensor enables measurements over a wide pH range without the use of multiple probes.
ISSN:	1530-6984 1530-6992
DOI:	10.1021/nl071418z