Expression and functional characterization of classical swine fever virus E(rns) protein

E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) with unusual RNase activity. Recently, E(rns) was found to have a new function of counteracting the beta-interferon (IFN-beta) induction pathway. In this study, wildtype ErnsSM and two mutated E(rns) proteins ErnsH297k and Erns...

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Veröffentlicht in:	Protein expression and purification 2007-10, Vol.55 (2), p.379-387
Hauptverfasser:	Chen, Liu, Xia, Yan-Hua, Pan, Zi-Shu, Zhang, Chu-Yu
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Blotting, Western Cell Line Classical Swine Fever - metabolism DNA Primers Escherichia coli - genetics Fluorescent Antibody Technique Interferon-beta - biosynthesis Ribonucleases - metabolism Viral Proteins - chemistry Viral Proteins - genetics Viral Proteins - metabolism
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Zusammenfassung:	E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) with unusual RNase activity. Recently, E(rns) was found to have a new function of counteracting the beta-interferon (IFN-beta) induction pathway. In this study, wildtype ErnsSM and two mutated E(rns) proteins ErnsH297k and ErnsH346k were expressed in insect cells and purified for RNase activity and function analysis. RNase activity assay in vitro demonstrated that only wildtype E(rns) protein had RNase activity. However, both wildtype ErnsSM and the two mutated E(rns)ErnsH297k and ErnsH346k as exogenous proteins had a block effect on Newcastle disease virus (NDV)-mediated IFN-beta promoter induction.
ISSN:	1046-5928
DOI:	10.1016/j.pep.2007.05.003