Increased apoptosis and decreased proliferation of colorectal cancer cells using insulin-like growth factor binding protein-4 gene delivered locally by gene transfer

Objective  Insulin‐like growth factor (IGF)‐I induces proliferation of transformed cells. Its binding proteins (IGFBP) are involved in local regulation of IGF. This study assessed the effects of overexpression of IGFBP‐4 on the development of cancer in vivo. Method  Nude mice were subcutaneously ino...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Colorectal disease 2007-09, Vol.9 (7), p.625-631
Hauptverfasser: Durai, R., Yang, S. Y., Sales, K. M., Seifalian, A. M., Goldspink, G., Winslet, M. C.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Objective  Insulin‐like growth factor (IGF)‐I induces proliferation of transformed cells. Its binding proteins (IGFBP) are involved in local regulation of IGF. This study assessed the effects of overexpression of IGFBP‐4 on the development of cancer in vivo. Method  Nude mice were subcutaneously inoculated with HT‐29 colorectal cancer cells (3 × 106). When the tumour became visible (1 week after inoculation), animals received either 150 μg of mammalian expression vector containing IGFBP‐4 cDNA or vector alone (n = 6 each) by peritumoural injection. Tumour size was measured during the growth. After 3 weeks of IGFBP‐4 induction, animals were killed and tumour tissue samples were collected for examining the level of IGFBP‐4 expression. Tumour mitotic activities were determined by counting numbers of mitotic cells on the tissue section. Apoptosis was investigated by terminal deoxynucleotidyl transferase‐mediated dUDP nick end labelling assay. Results  Following IGFBP‐4 treatment, tumour showed large necrotic areas, significantly increased numbers of apoptotic cells (36.67 ± 7.36 vs 7.07 ± 1.91, P 
ISSN:1462-8910
1463-1318
DOI:10.1111/j.1463-1318.2006.01190.x