Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection

Identification of individuals with a tuberculosis infection is a very important element for the control of tuberculosis. The currently used tuberculin skin test has poor sensitivity and specificity. Recently, an important advance in tuberculosis diagnosis occurred with the development of in vitro T...

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Veröffentlicht in:	The New microbiologica 2007-07, Vol.30 (3), p.291-294
Hauptverfasser:	Mantegani, Paola, Piana, Federica, Galli, Laura, Scarpellini, Paolo, Codecasa, Luigi, Lazzarin, Adriano, Cirillo, Daniela, Fortis, Claudio
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aged Aged, 80 and over Bacterial Proteins - immunology Child Child, Preschool Female Humans Immunoenzyme Techniques - methods Infant Interferon-gamma - biosynthesis Male Middle Aged Mycobacterium tuberculosis Mycobacterium tuberculosis - metabolism Peptides - chemical synthesis Peptides - immunology Reagent Kits, Diagnostic Sensitivity and Specificity T-Lymphocytes - immunology Tuberculosis - blood Tuberculosis - diagnosis
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Beschreibung
Zusammenfassung:	Identification of individuals with a tuberculosis infection is a very important element for the control of tuberculosis. The currently used tuberculin skin test has poor sensitivity and specificity. Recently, an important advance in tuberculosis diagnosis occurred with the development of in vitro T cell-based IFN-gamma release assays. The aim of this study was to compare a RD1-based in-house ELISPOT-IFN-gamma assay with a commercial (T-SPOT.TB) assay for the diagnosis of tuberculosis infection. The results showed an almost complete concordance between the two assays, confirming that our restricted but highly selected pool of peptides is sufficient to detect tuberculosis infection.
ISSN:	1121-7138