Microbial contamination of BM products before and after processing: a report of incidence and immediate adverse events in 257 grafts
Background The incidence and potential clinical consequences of bacterial contamination of autologous and allogeneic BM products remains open to question. We report our experience of bacterial contamination of BM grafts and adverse events that occurred after transplantation. Methods From January 200...
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| Veröffentlicht in: | Cytotherapy (Oxford, England) England), 2007, Vol.9 (5), p.508-513 |
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| creator | Vanneaux, V Foïs, E Robin, M Rea, D Peffault de Latour, R Biscay, N Chantre, E Robert, I Wargnier, A Traineau, R Benbunan, M Marolleau, J.P Socié, G Larghero, J |
| description | Background The incidence and potential clinical consequences of bacterial contamination of autologous and allogeneic BM products remains open to question. We report our experience of bacterial contamination of BM grafts and adverse events that occurred after transplantation. Methods From January 2003 to February 2006, 257 BM harvests were processed and infused at our institution. Analysis of microbial contamination incidence before and after processing, sensitivity spectra of isolated bacteria and adverse events after graft infusion were analyzed. Results Nineteen of 257 BM (7.4%) were contaminated. Coagulase-negative Staphylococcus ( n = 9) and Propionibacterium acnes ( n = 6) were the most frequently isolated microorganisms. Two of nine coagulase-negative staphylococci were found to be resistant to erythromycin and two of six P. acnes to fosfomycin and gentamycin. The frequency and severity of immediate adverse events reported in patients receiving a contaminated graft were similar to those observed in patients receiving a non-contaminated product. No major adverse sequelae occurred after infusion of contaminated grafts. Finally, none of the patients transplanted with a contaminated graft developed bacteriemia that could have been related to the isolated microorganism. Discussion Microbial contamination of BM progenitor cell grafts does not induce severe clinical complications or infectious diseases after infusion. The vast majority of isolated pathogens were skin contaminants. |
| doi_str_mv | 10.1080/14653240701420427 |
| format | Article |
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We report our experience of bacterial contamination of BM grafts and adverse events that occurred after transplantation. Methods From January 2003 to February 2006, 257 BM harvests were processed and infused at our institution. Analysis of microbial contamination incidence before and after processing, sensitivity spectra of isolated bacteria and adverse events after graft infusion were analyzed. Results Nineteen of 257 BM (7.4%) were contaminated. Coagulase-negative Staphylococcus ( n = 9) and Propionibacterium acnes ( n = 6) were the most frequently isolated microorganisms. Two of nine coagulase-negative staphylococci were found to be resistant to erythromycin and two of six P. acnes to fosfomycin and gentamycin. The frequency and severity of immediate adverse events reported in patients receiving a contaminated graft were similar to those observed in patients receiving a non-contaminated product. No major adverse sequelae occurred after infusion of contaminated grafts. Finally, none of the patients transplanted with a contaminated graft developed bacteriemia that could have been related to the isolated microorganism. Discussion Microbial contamination of BM progenitor cell grafts does not induce severe clinical complications or infectious diseases after infusion. The vast majority of isolated pathogens were skin contaminants.</description><identifier>ISSN: 1465-3249</identifier><identifier>EISSN: 1477-2566</identifier><identifier>DOI: 10.1080/14653240701420427</identifier><identifier>PMID: 17786612</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Advanced Basic Science ; adverse events ; Anti-Infective Agents, Local - therapeutic use ; Bacterial Infections - etiology ; Bacterial Infections - prevention & control ; BMT ; Bone Marrow Transplantation - adverse effects ; Bone Marrow Transplantation - standards ; Child ; Child, Preschool ; Female ; graft contamination ; graft processing ; Humans ; Incidence ; Infant ; Male ; Middle Aged ; Other ; Propionibacterium acnes ; Retrospective Studies ; Skin - microbiology ; Staphylococcus ; Stem Cells - microbiology ; Surgical Wound Infection - epidemiology ; Surgical Wound Infection - microbiology ; Surgical Wound Infection - prevention & control ; Transplantation, Autologous - adverse effects ; Transplantation, Autologous - standards ; Transplantation, Homologous - adverse effects ; Transplantation, Homologous - standards</subject><ispartof>Cytotherapy (Oxford, England), 2007, Vol.9 (5), p.508-513</ispartof><rights>International Society for Cellular Therapy</rights><rights>2007 International Society for Cellular Therapy</rights><rights>2007 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-717e6639fdb7eb24c2eb713688b785c8807a1a4920ec403b5170334a96be64d73</citedby><cites>FETCH-LOGICAL-c489t-717e6639fdb7eb24c2eb713688b785c8807a1a4920ec403b5170334a96be64d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/14653240701420427$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/14653240701420427$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,61221,61402</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17786612$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vanneaux, V</creatorcontrib><creatorcontrib>Foïs, E</creatorcontrib><creatorcontrib>Robin, M</creatorcontrib><creatorcontrib>Rea, D</creatorcontrib><creatorcontrib>Peffault de Latour, R</creatorcontrib><creatorcontrib>Biscay, N</creatorcontrib><creatorcontrib>Chantre, E</creatorcontrib><creatorcontrib>Robert, I</creatorcontrib><creatorcontrib>Wargnier, A</creatorcontrib><creatorcontrib>Traineau, R</creatorcontrib><creatorcontrib>Benbunan, M</creatorcontrib><creatorcontrib>Marolleau, J.P</creatorcontrib><creatorcontrib>Socié, G</creatorcontrib><creatorcontrib>Larghero, J</creatorcontrib><title>Microbial contamination of BM products before and after processing: a report of incidence and immediate adverse events in 257 grafts</title><title>Cytotherapy (Oxford, England)</title><addtitle>Cytotherapy</addtitle><description>Background The incidence and potential clinical consequences of bacterial contamination of autologous and allogeneic BM products remains open to question. We report our experience of bacterial contamination of BM grafts and adverse events that occurred after transplantation. Methods From January 2003 to February 2006, 257 BM harvests were processed and infused at our institution. Analysis of microbial contamination incidence before and after processing, sensitivity spectra of isolated bacteria and adverse events after graft infusion were analyzed. Results Nineteen of 257 BM (7.4%) were contaminated. Coagulase-negative Staphylococcus ( n = 9) and Propionibacterium acnes ( n = 6) were the most frequently isolated microorganisms. Two of nine coagulase-negative staphylococci were found to be resistant to erythromycin and two of six P. acnes to fosfomycin and gentamycin. The frequency and severity of immediate adverse events reported in patients receiving a contaminated graft were similar to those observed in patients receiving a non-contaminated product. No major adverse sequelae occurred after infusion of contaminated grafts. Finally, none of the patients transplanted with a contaminated graft developed bacteriemia that could have been related to the isolated microorganism. Discussion Microbial contamination of BM progenitor cell grafts does not induce severe clinical complications or infectious diseases after infusion. The vast majority of isolated pathogens were skin contaminants.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Advanced Basic Science</subject><subject>adverse events</subject><subject>Anti-Infective Agents, Local - therapeutic use</subject><subject>Bacterial Infections - etiology</subject><subject>Bacterial Infections - prevention & control</subject><subject>BMT</subject><subject>Bone Marrow Transplantation - adverse effects</subject><subject>Bone Marrow Transplantation - standards</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>graft contamination</subject><subject>graft processing</subject><subject>Humans</subject><subject>Incidence</subject><subject>Infant</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Other</subject><subject>Propionibacterium acnes</subject><subject>Retrospective Studies</subject><subject>Skin - microbiology</subject><subject>Staphylococcus</subject><subject>Stem Cells - microbiology</subject><subject>Surgical Wound Infection - epidemiology</subject><subject>Surgical Wound Infection - microbiology</subject><subject>Surgical Wound Infection - prevention & control</subject><subject>Transplantation, Autologous - adverse effects</subject><subject>Transplantation, Autologous - standards</subject><subject>Transplantation, Homologous - adverse effects</subject><subject>Transplantation, Homologous - standards</subject><issn>1465-3249</issn><issn>1477-2566</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9rFTEUxQdRbK1-ADeSlbvR_E9GQajFVqHFhboOmcydNnUmeSaZB937wc3wBgoKdZUb7jm_hHOa5iXBbwjW-C3hUjDKscKEU8ypetQcE65US4WUj9dZirYKuqPmWc63GFOstXjaHBGltJSEHje_r7xLsfd2Qi6GYmcfbPExoDiij1dol-KwuJJRD2NMgGwYkB0LpHXjIGcfrt8hixLsYiqryQfnBwjuoPXzDIO3pd6GPaQMCPYQKs8HRIVC16nS8vPmyWinDC-286T5cf7p-9nn9vLrxZez08vWcd2VVhEFUrJuHHoFPeWOQq8Ik1r3SgunNVaWWN5RDI5j1guiMGPcdrIHyQfFTprXB279_K8FcjGzzw6myQaISzZSU8Y0lv8VUkypwGolkoOwhphzgtHskp9tujMEm7Uj809H1fNqgy99TefesZVSBe8PAh9q6LO9ATuVG2cTmNu4pFAjehC_uaEmufeQTHZ-LWTwCVwxQ_QPuj_85XaTD97Z6SfcQb5_32RqsPm2EboaBiaECfYH97XFlw</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Vanneaux, V</creator><creator>Foïs, E</creator><creator>Robin, M</creator><creator>Rea, D</creator><creator>Peffault de Latour, R</creator><creator>Biscay, N</creator><creator>Chantre, E</creator><creator>Robert, I</creator><creator>Wargnier, A</creator><creator>Traineau, R</creator><creator>Benbunan, M</creator><creator>Marolleau, J.P</creator><creator>Socié, G</creator><creator>Larghero, J</creator><general>Elsevier Inc</general><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2007</creationdate><title>Microbial contamination of BM products before and after processing: a report of incidence and immediate adverse events in 257 grafts</title><author>Vanneaux, V ; 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We report our experience of bacterial contamination of BM grafts and adverse events that occurred after transplantation. Methods From January 2003 to February 2006, 257 BM harvests were processed and infused at our institution. Analysis of microbial contamination incidence before and after processing, sensitivity spectra of isolated bacteria and adverse events after graft infusion were analyzed. Results Nineteen of 257 BM (7.4%) were contaminated. Coagulase-negative Staphylococcus ( n = 9) and Propionibacterium acnes ( n = 6) were the most frequently isolated microorganisms. Two of nine coagulase-negative staphylococci were found to be resistant to erythromycin and two of six P. acnes to fosfomycin and gentamycin. The frequency and severity of immediate adverse events reported in patients receiving a contaminated graft were similar to those observed in patients receiving a non-contaminated product. No major adverse sequelae occurred after infusion of contaminated grafts. Finally, none of the patients transplanted with a contaminated graft developed bacteriemia that could have been related to the isolated microorganism. Discussion Microbial contamination of BM progenitor cell grafts does not induce severe clinical complications or infectious diseases after infusion. The vast majority of isolated pathogens were skin contaminants.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>17786612</pmid><doi>10.1080/14653240701420427</doi><tpages>6</tpages></addata></record> |
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| subjects | Adolescent Adult Advanced Basic Science adverse events Anti-Infective Agents, Local - therapeutic use Bacterial Infections - etiology Bacterial Infections - prevention & control BMT Bone Marrow Transplantation - adverse effects Bone Marrow Transplantation - standards Child Child, Preschool Female graft contamination graft processing Humans Incidence Infant Male Middle Aged Other Propionibacterium acnes Retrospective Studies Skin - microbiology Staphylococcus Stem Cells - microbiology Surgical Wound Infection - epidemiology Surgical Wound Infection - microbiology Surgical Wound Infection - prevention & control Transplantation, Autologous - adverse effects Transplantation, Autologous - standards Transplantation, Homologous - adverse effects Transplantation, Homologous - standards |
| title | Microbial contamination of BM products before and after processing: a report of incidence and immediate adverse events in 257 grafts |
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