Humihabitans oryzae gen. nov., sp. nov

1 Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan 2 The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan Correspondence Yoko Takahashi ytakaha{at}lisci.kitasato-u.ac.jp Strain KV-657 T was isolated from a paddy field soil sample collected in Japan using GPM agar plates supplemented with catalase. The strain was a Gram-positive, aerobic organism that formed branching hyphae with LL -diaminopimelic acid as the diagnostic peptidoglycan diamino acid. The major menaquinone was MK-8(H 4 ). Mycolic acids were not detected. The G+C content of the DNA was 70 mol%. 16S rRNA gene sequence analysis revealed that this strain is closely related to Intrasporangium calvum DSM 43043 T , with a similarity of 97.6 %. Based on the morphological, biochemical and chemotaxonomic properties of this strain and phylogenetic analysis, it was concluded that this isolate represents a new genus and species in the family Intrasporangiaceae , for which the name Humihabitans oryzae gen. nov., sp. nov. is proposed. The type strain of Humihabitans oryzae is KV-657 T (=NRRL B-24470 T =NBRC 101802 T ). Abbreviations: A 2 pm, diaminopimelic acid The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KV-657 T is AB282887 . Scanning electron micrographs of cells of strain KV-657 T and HPLC traces from menaquinone analysis of the novel strain and I. calvum NBRC 12989 T are available as supplementary material with the online version of this paper.