Bone marrow derived mast cell acquire responsiveness to substance P with Ca(2+) signals and release of leukotriene B(4) via mitogen-activated protein kinase

Substance P (SP) selectively activates mast cells that reside in connective tissues. We studied the reactions of bone marrow-derived mast cells (BMMC) of three mouse strains, cultured with or without fibroblasts. BMMC co-cultured with fibroblasts, but not those cultured alone, increased intracellula...

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Veröffentlicht in:	Journal of neuroimmunology 2006-12, Vol.181 (1-2), p.1-12
Hauptverfasser:	Okabe, Tsutomu, Hide, Michihiro, Hiragun, Takaaki, Morita, Eishin, Koro, Osamu, Yamamoto, Shoso
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Bone Marrow Cells - metabolism Calcium - metabolism Calcium Signaling - drug effects Calcium Signaling - immunology Coculture Techniques Enzyme Inhibitors - pharmacology Fibroblasts - cytology Flavonoids - pharmacology Histamine Release - drug effects Imidazoles - pharmacology Leukotriene B4 - metabolism Male Mast Cells - cytology Mast Cells - drug effects Mast Cells - metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Mitogen-Activated Protein Kinases - antagonists & inhibitors Mitogen-Activated Protein Kinases - metabolism NIH 3T3 Cells Pyridines - pharmacology Species Specificity Substance P - immunology Substance P - metabolism Substance P - pharmacology
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Zusammenfassung:	Substance P (SP) selectively activates mast cells that reside in connective tissues. We studied the reactions of bone marrow-derived mast cells (BMMC) of three mouse strains, cultured with or without fibroblasts. BMMC co-cultured with fibroblasts, but not those cultured alone, increased intracellular Ca(2+), released LTB(4) and histamine in response to SP. PD098059 significantly inhibited the release of LTB(4), but not histamine in all strains. SB203580 failed to reduce or slightly impaired the release of LTB(4). These results suggest that mast cells undergo maturation under the influence of fibroblasts, acquiring the responsiveness to SP with Ca(2+) signals and predominantly ERK-MAP kinase.
ISSN:	0165-5728