Development of a NACE method for simultaneous measurement of three adenosine monophosphate isomers in biomimicking prebiotic synthesis without sample pretreatment

A practical NACE method was developed for simultaneous determination of three adenosine monophosphate (AMP) isomers. Separation of three AMP isomers was achieved using 200 mM Tris/H3BO3 in acetontrile/water (2:1 v/v) at pH* 10.0 as the running buffer and +25 kV as the applied voltage over a bare fused‐silica capillary of 50 µm id×375 µm od×54.5 cm (46 cm to the detector window). At 260 nm, the calibration curves were linear in the range of 1–100 µg/mL. The detection limits were less than 0.70 µg/mL. The recovery ranged from 94.5 to 106.4%. The intraday RSDs of the migration times were between 2.1 and 3.0%. The developed NACE method has been successfully applied for the determination of three AMP isomers in the real samples of biomimicking prebiotic synthesis reaction between N‐(O,O‐diisopropyl) phosphoryl amino acid and adenosine.