Quantification of Circulating Plasma DNA Fragments as Tumor Markers in Patients with Esophageal Cancer

The quantity and quality of circulating DNA fragments was analyzed by quantitative real-time polymerase chain reactions (qPCR) in plasma from patients with esophageal carcinomas, in order to assess their diagnostic value. Patients and Methods: Plasma was collected preoperatively from 24 patients wit...

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Veröffentlicht in:	Anticancer research 2007-07, Vol.27 (4C), p.2737-2741
Hauptverfasser:	TOMITA, Haruhisa, ICHIKAWA, Daisuke, OTSUJI, Eigo, IKOMA, Daito, SAI, Soujin, TANI, Nobuyuki, IKOMA, Hisashi, FUJIWARA, Hitoshi, KIKUCHI, Shojiro, OKAMOTO, Kazuma, OCHIAI, Toshiya
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Biological and medical sciences Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Carcinoma, Squamous Cell - blood Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - pathology Case-Control Studies DNA, Neoplasm - blood Esophageal Neoplasms - blood Esophageal Neoplasms - genetics Esophageal Neoplasms - pathology Esophagus Gastroenterology. Liver. Pancreas. Abdomen Humans Medical sciences Middle Aged Neoplasm Staging Polymerase Chain Reaction - methods ROC Curve Tumors
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Zusammenfassung:	The quantity and quality of circulating DNA fragments was analyzed by quantitative real-time polymerase chain reactions (qPCR) in plasma from patients with esophageal carcinomas, in order to assess their diagnostic value. Patients and Methods: Plasma was collected preoperatively from 24 patients with esophageal cancer and 21 healthy controls. qPCR was performed using two primer sets for the beta-actin gene, amplifying short and long segments. Results: The DNA concentrations in both the short and long assays of esophageal cancer patients were significantly higher than the controls (p
ISSN:	0250-7005 1791-7530