The effectiveness of a CpG motif-based adjuvant (CpG ODN 2006) for LHRH immunization

Abstract A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum . Also, the immuno...

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Veröffentlicht in:	Vaccine 2007-08, Vol.25 (35), p.6537-6543
Hauptverfasser:	Conforti, Valéria A, de Avila, David M, Cummings, Nada S, Wells, Kenny J, Ülker, Hasan, Reeves, Jerry J
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates Adjuvants, Immunologic - chemistry Adjuvants, Immunologic - pharmacology Allergy and Immunology Animals Antibodies - analysis Antigens Antigens - chemistry Antigens - immunology Contraception, Immunologic - methods CpG ODN Deoxyribonucleic acid DNA E coli Escherichia coli - immunology Female Freeze Drying Gonadotropin-Releasing Hormone - immunology Hemodialysis Hormones Immunization Immunogenicity Laboratories LHRH immunization Mammals Methylene Chloride Mycobacterium Mycobacterium - immunology Mycobacterium butyricum Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - pharmacology Organ Size - drug effects Organic solvents Ovalbumin - immunology Ovary - anatomy & histology Ovary - drug effects Polymers Proteins Rats Rats, Sprague-Dawley Rodents Solvents Urea Vaccines - chemical synthesis Vaccines - immunology
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Zusammenfassung:	Abstract A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum . Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2007.05.056