A new time-resolved fluorometric microarray detection system using core-shell-type fluorescent nanosphere and its application to allergen microarray

We have developed a new time-resolved fluorometric (TRF) microarray detection system consisting of fluorescent NH2 nanosphere, TRF microarray detector and gamma-irradiated polystyrene chip. Using the TRF microarray detector, we detected 500 particles of the fluorescent nanosphere in one channel. Cro...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2006-07, Vol.385 (5), p.797-806
Hauptverfasser:	Matsuya, Takeshi, Otake, Kazuhiko, Tashiro, Shigeru, Hoshino, Nobuhiro, Katada, Motomi, Okuyama, Tsuneo
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergens - analysis Allergens - immunology Fluorometry Humans Immunoglobulin E - blood Immunoglobulin E - immunology Nanotubes - chemistry Nanotubes - radiation effects Polystyrenes - chemistry Protein Array Analysis - instrumentation Protein Array Analysis - methods Reproducibility of Results Time Factors
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Beschreibung
Zusammenfassung:	We have developed a new time-resolved fluorometric (TRF) microarray detection system consisting of fluorescent NH2 nanosphere, TRF microarray detector and gamma-irradiated polystyrene chip. Using the TRF microarray detector, we detected 500 particles of the fluorescent nanosphere in one channel. Cross-talk fluorescence from the adjacent channels was little observed in the TRF microarray detector (
ISSN:	1618-2642 1618-2650
DOI:	10.1007/s00216-006-0455-9