Development and application of a high-performance liquid chromatography–mass spectrometry method to determine alendronate in human urine
Despite the high potential offered by electrospray ionization on highly polar compounds like biphosphonates, few applications have been developed. High-performance liquid chromatography (HPLC) separation methods suitable for such molecules cannot be used in tandem with mass spectrometry (MS) due to...
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Veröffentlicht in: | Journal of Chromatography A 2007-08, Vol.1160 (1), p.21-33 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Despite the high potential offered by electrospray ionization on highly polar compounds like biphosphonates, few applications have been developed. High-performance liquid chromatography (HPLC) separation methods suitable for such molecules cannot be used in tandem with mass spectrometry (MS) due to high non-volatile salt content; at the same time the sample preparation, in biological fluids, is also a challenging problem. In the past ion-pair chromatography was mainly used in the case of HPLC–MS of biphosphonates, but no application to quantitative pharmacokinetic (PK) studies has been presented. In this study, after preliminary tests with ion-pair chromatography showing a poor sensitivity, a combined derivatization of the amino group and the biphosphonate has been developed and tested in a PK study. Using this analytical approach we were able to fully validate the quantitation of alendronate in the range of 6.667–4860.0
ng/ml in urine (sample volume 2.0
ml); each analytical run was 5.0
min long. The sensitivity achieved permitted a correct evaluation of the alendronate urinary excretion over the full period of urine collection. Sample preparation despite its complexity permitted to process and analyze up to 200 samples in a working day. |
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ISSN: | 0021-9673 |
DOI: | 10.1016/j.chroma.2007.04.020 |