Self-Cleavable Bioluminogenic Luciferin Phosphates as Alkaline Phosphatase Reporters

Self-Cleavable Bioluminogenic Luciferin Phosphates as Alkaline Phosphatase Reporters

After glow. Reactive chemical adaptors were used to stabilize bioluminogenic phosphates for monitoring alkaline phosphatase (AP) activity (see scheme). The 1,6‐elimination based luciferin phosphate exhibited ultrasensitive ability to detect ∼10−22 mol of AP enzyme in a homogeneous solution, and pico...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2008-03, Vol.9 (5), p.714-718
Hauptverfasser: Zhou, Wenhui, Andrews, Christine, Liu, Jianquan, Shultz, John W, Valley, Michael P, Cali, Jim J, Hawkins, Erika M, Klaubert, Dieter H, Bulleit, Robert F, Wood, Keith V
Format: Artikel
Sprache:eng
Schlagworte:
alkaline phosphatase
Alkaline Phosphatase - analysis
Alkaline Phosphatase - chemistry
Firefly Luciferin - chemical synthesis
Firefly Luciferin - chemistry
immunoassays
Luminescence
Molecular Structure
Phosphates - chemical synthesis
Phosphates - chemistry
self-cleavable adaptors
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Zusammenfassung:After glow. Reactive chemical adaptors were used to stabilize bioluminogenic phosphates for monitoring alkaline phosphatase (AP) activity (see scheme). The 1,6‐elimination based luciferin phosphate exhibited ultrasensitive ability to detect ∼10−22 mol of AP enzyme in a homogeneous solution, and picograms of protein in an immunoassay.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200700644