Alpha2-adrenergic receptor subtype-specific activation of NF-kappaB in PC12 cells
In the present study we sought to investigate the signal transduction mechanisms that underlie the alpha2-adrenergic receptor (AR)-induced, subtype-specific neuronal differentiation of PC12 cells. Alpha2-ARs induced NF-kappaB transcriptional activity and p21(waf-1) gene transcription in the same sub...
Gespeichert in:
| Veröffentlicht in: | Neuroscience letters 2006-07, Vol.402 (3), p.210-215 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 215 |
|---|---|
| container_issue | 3 |
| container_start_page | 210 |
| container_title | Neuroscience letters |
| container_volume | 402 |
| creator | Lymperopoulos, Anastasios Karkoulias, Georgios Koch, Walter J Flordellis, Christodoulos S |
| description | In the present study we sought to investigate the signal transduction mechanisms that underlie the alpha2-adrenergic receptor (AR)-induced, subtype-specific neuronal differentiation of PC12 cells. Alpha2-ARs induced NF-kappaB transcriptional activity and p21(waf-1) gene transcription in the same subtype-specific manner (alpha2A |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_68070850</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68070850</sourcerecordid><originalsourceid>FETCH-LOGICAL-p540-7b74e2f1f35edb0df43d0f11df792253540ee0f62faf7e611a3451ab5b77b05d3</originalsourceid><addsrcrecordid>eNo1kM1KAzEYRbNQbK2-gmTlLvDlb9Iua7FaKP5A90My-aLR6UxMZoS-vRXr6i7u4XC5Z2QKEhSTCwUTclnKBwBortUFmfDKSOACpuR12aZ3K5j1GTvMb7GhGRtMQ59pGd1wSMhKwiaGY2ObIX7bIfYd7QN9WrNPm5K9o7GjLysuaINtW67IebBtwetTzshufb9bPbLt88NmtdyypBUw44xCEXiQGr0DH5T0EDj3wSyE0PLIIEKoRLDBYMW5lUpz67QzxoH2ckZu_7Qp918jlqHex_I7wHbYj6Wu5mBgruEI3pzA0e3R1ynHvc2H-v8D-QMMBFZs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68070850</pqid></control><display><type>article</type><title>Alpha2-adrenergic receptor subtype-specific activation of NF-kappaB in PC12 cells</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Lymperopoulos, Anastasios ; Karkoulias, Georgios ; Koch, Walter J ; Flordellis, Christodoulos S</creator><creatorcontrib>Lymperopoulos, Anastasios ; Karkoulias, Georgios ; Koch, Walter J ; Flordellis, Christodoulos S</creatorcontrib><description>In the present study we sought to investigate the signal transduction mechanisms that underlie the alpha2-adrenergic receptor (AR)-induced, subtype-specific neuronal differentiation of PC12 cells. Alpha2-ARs induced NF-kappaB transcriptional activity and p21(waf-1) gene transcription in the same subtype-specific manner (alpha2A<alpha2B<alpha2C) as the neuronal differentiation induced by these receptors. Following pretreatment with the MEK1 inhibitor PD98059, the NF-kappaB response to epinephrine becomes uniform with loss of subtype specificity. In contrast, there is complete abolishment of epinephrine-induced transcription by NF-kappaB after pretreatment with the PI3-K inhibitor LY294002. These data imply that induction of transcription by NF-kappaB is PI-3K-dependent; however, the subtype-specific induction of transcription by NF-kappaB is ERK1/2-dependent. Taken together, these results suggest that the three alpha2-adrenoceptors promote an interaction between PI-3K and ERK1/2 in a subtype-specific way, leading to subtype-specific induction of transcription by NF-kappaB and p21(waf-1) gene transcription, which might underlie the subtype-specific differentiation of PC12 cells induced by these receptors.</description><identifier>ISSN: 0304-3940</identifier><identifier>PMID: 16730120</identifier><language>eng</language><publisher>Ireland</publisher><subject>Animals ; Cell Differentiation ; Cyclin-Dependent Kinase Inhibitor p21 - genetics ; Humans ; Mitogen-Activated Protein Kinase 1 - physiology ; Mitogen-Activated Protein Kinase 3 - physiology ; NF-kappa B - genetics ; NF-kappa B - metabolism ; PC12 Cells ; Phosphatidylinositol 3-Kinases - physiology ; Rats ; Receptors, Adrenergic, alpha-2 - physiology ; Signal Transduction ; Transcription, Genetic</subject><ispartof>Neuroscience letters, 2006-07, Vol.402 (3), p.210-215</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16730120$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lymperopoulos, Anastasios</creatorcontrib><creatorcontrib>Karkoulias, Georgios</creatorcontrib><creatorcontrib>Koch, Walter J</creatorcontrib><creatorcontrib>Flordellis, Christodoulos S</creatorcontrib><title>Alpha2-adrenergic receptor subtype-specific activation of NF-kappaB in PC12 cells</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>In the present study we sought to investigate the signal transduction mechanisms that underlie the alpha2-adrenergic receptor (AR)-induced, subtype-specific neuronal differentiation of PC12 cells. Alpha2-ARs induced NF-kappaB transcriptional activity and p21(waf-1) gene transcription in the same subtype-specific manner (alpha2A<alpha2B<alpha2C) as the neuronal differentiation induced by these receptors. Following pretreatment with the MEK1 inhibitor PD98059, the NF-kappaB response to epinephrine becomes uniform with loss of subtype specificity. In contrast, there is complete abolishment of epinephrine-induced transcription by NF-kappaB after pretreatment with the PI3-K inhibitor LY294002. These data imply that induction of transcription by NF-kappaB is PI-3K-dependent; however, the subtype-specific induction of transcription by NF-kappaB is ERK1/2-dependent. Taken together, these results suggest that the three alpha2-adrenoceptors promote an interaction between PI-3K and ERK1/2 in a subtype-specific way, leading to subtype-specific induction of transcription by NF-kappaB and p21(waf-1) gene transcription, which might underlie the subtype-specific differentiation of PC12 cells induced by these receptors.</description><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Cyclin-Dependent Kinase Inhibitor p21 - genetics</subject><subject>Humans</subject><subject>Mitogen-Activated Protein Kinase 1 - physiology</subject><subject>Mitogen-Activated Protein Kinase 3 - physiology</subject><subject>NF-kappa B - genetics</subject><subject>NF-kappa B - metabolism</subject><subject>PC12 Cells</subject><subject>Phosphatidylinositol 3-Kinases - physiology</subject><subject>Rats</subject><subject>Receptors, Adrenergic, alpha-2 - physiology</subject><subject>Signal Transduction</subject><subject>Transcription, Genetic</subject><issn>0304-3940</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kM1KAzEYRbNQbK2-gmTlLvDlb9Iua7FaKP5A90My-aLR6UxMZoS-vRXr6i7u4XC5Z2QKEhSTCwUTclnKBwBortUFmfDKSOACpuR12aZ3K5j1GTvMb7GhGRtMQ59pGd1wSMhKwiaGY2ObIX7bIfYd7QN9WrNPm5K9o7GjLysuaINtW67IebBtwetTzshufb9bPbLt88NmtdyypBUw44xCEXiQGr0DH5T0EDj3wSyE0PLIIEKoRLDBYMW5lUpz67QzxoH2ckZu_7Qp918jlqHex_I7wHbYj6Wu5mBgruEI3pzA0e3R1ynHvc2H-v8D-QMMBFZs</recordid><startdate>20060724</startdate><enddate>20060724</enddate><creator>Lymperopoulos, Anastasios</creator><creator>Karkoulias, Georgios</creator><creator>Koch, Walter J</creator><creator>Flordellis, Christodoulos S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20060724</creationdate><title>Alpha2-adrenergic receptor subtype-specific activation of NF-kappaB in PC12 cells</title><author>Lymperopoulos, Anastasios ; Karkoulias, Georgios ; Koch, Walter J ; Flordellis, Christodoulos S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p540-7b74e2f1f35edb0df43d0f11df792253540ee0f62faf7e611a3451ab5b77b05d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Cyclin-Dependent Kinase Inhibitor p21 - genetics</topic><topic>Humans</topic><topic>Mitogen-Activated Protein Kinase 1 - physiology</topic><topic>Mitogen-Activated Protein Kinase 3 - physiology</topic><topic>NF-kappa B - genetics</topic><topic>NF-kappa B - metabolism</topic><topic>PC12 Cells</topic><topic>Phosphatidylinositol 3-Kinases - physiology</topic><topic>Rats</topic><topic>Receptors, Adrenergic, alpha-2 - physiology</topic><topic>Signal Transduction</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lymperopoulos, Anastasios</creatorcontrib><creatorcontrib>Karkoulias, Georgios</creatorcontrib><creatorcontrib>Koch, Walter J</creatorcontrib><creatorcontrib>Flordellis, Christodoulos S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lymperopoulos, Anastasios</au><au>Karkoulias, Georgios</au><au>Koch, Walter J</au><au>Flordellis, Christodoulos S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alpha2-adrenergic receptor subtype-specific activation of NF-kappaB in PC12 cells</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2006-07-24</date><risdate>2006</risdate><volume>402</volume><issue>3</issue><spage>210</spage><epage>215</epage><pages>210-215</pages><issn>0304-3940</issn><abstract>In the present study we sought to investigate the signal transduction mechanisms that underlie the alpha2-adrenergic receptor (AR)-induced, subtype-specific neuronal differentiation of PC12 cells. Alpha2-ARs induced NF-kappaB transcriptional activity and p21(waf-1) gene transcription in the same subtype-specific manner (alpha2A<alpha2B<alpha2C) as the neuronal differentiation induced by these receptors. Following pretreatment with the MEK1 inhibitor PD98059, the NF-kappaB response to epinephrine becomes uniform with loss of subtype specificity. In contrast, there is complete abolishment of epinephrine-induced transcription by NF-kappaB after pretreatment with the PI3-K inhibitor LY294002. These data imply that induction of transcription by NF-kappaB is PI-3K-dependent; however, the subtype-specific induction of transcription by NF-kappaB is ERK1/2-dependent. Taken together, these results suggest that the three alpha2-adrenoceptors promote an interaction between PI-3K and ERK1/2 in a subtype-specific way, leading to subtype-specific induction of transcription by NF-kappaB and p21(waf-1) gene transcription, which might underlie the subtype-specific differentiation of PC12 cells induced by these receptors.</abstract><cop>Ireland</cop><pmid>16730120</pmid><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 2006-07, Vol.402 (3), p.210-215 |
| issn | 0304-3940 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68070850 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Cell Differentiation Cyclin-Dependent Kinase Inhibitor p21 - genetics Humans Mitogen-Activated Protein Kinase 1 - physiology Mitogen-Activated Protein Kinase 3 - physiology NF-kappa B - genetics NF-kappa B - metabolism PC12 Cells Phosphatidylinositol 3-Kinases - physiology Rats Receptors, Adrenergic, alpha-2 - physiology Signal Transduction Transcription, Genetic |
| title | Alpha2-adrenergic receptor subtype-specific activation of NF-kappaB in PC12 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T13%3A27%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Alpha2-adrenergic%20receptor%20subtype-specific%20activation%20of%20NF-kappaB%20in%20PC12%20cells&rft.jtitle=Neuroscience%20letters&rft.au=Lymperopoulos,%20Anastasios&rft.date=2006-07-24&rft.volume=402&rft.issue=3&rft.spage=210&rft.epage=215&rft.pages=210-215&rft.issn=0304-3940&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E68070850%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68070850&rft_id=info:pmid/16730120&rfr_iscdi=true |