Immaturity of IL‐18 gene expression and protein production in cord blood (CB) versus peripheral blood (PB) mononuclear cells and differential effects in natural killer (NK) cell development and function

Immaturity of IL‐18 gene expression and protein production in cord blood (CB) versus peripheral blood (PB) mononuclear cells and differential effects in natural killer (NK) cell development and function

Summary We have previously demonstrated dysregulation of IL‐12 and IL‐15 gene and protein expression between activated cord blood (CB) versus peripheral blood (PB) mononuclear cells (MNCs). In the present study, we compared IL‐18 gene expression and protein production and IL‐18 mRNA half‐life in bas...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:British journal of haematology 2005-07, Vol.130 (2), p.284-292
Hauptverfasser: Satwani, Prakash, Ayello, Janet, Ven, Carmella, O'Neill, Allison F., Simpson, Lynn L., Baxi, Laxmi, Cairo, Mitchell S.
Format: Artikel
Sprache:eng
Schlagworte:
Apoptosis
Biological and medical sciences
Cell Division
Cell Survival
Cells, Cultured
cord blood
Cytotoxicity, Immunologic
Fetal Blood - immunology
Gene Expression
Half-Life
Hematologic and hematopoietic diseases
Hematology
Humans
Interferon-gamma - biosynthesis
interferon‐gamma
Interleukin-12 - immunology
Interleukin-18 - biosynthesis
Interleukin-18 - genetics
Interleukin-18 - immunology
interleukin‐18
Killer Cells, Natural - cytology
Killer Cells, Natural - immunology
Leukocytes, Mononuclear - immunology
Medical sciences
natural killer cells
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - blood
RNA, Messenger - genetics
stem cell transplant
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Summary We have previously demonstrated dysregulation of IL‐12 and IL‐15 gene and protein expression between activated cord blood (CB) versus peripheral blood (PB) mononuclear cells (MNCs). In the present study, we compared IL‐18 gene expression and protein production and IL‐18 mRNA half‐life in basal versus activated CB versus PB MNCs, the effects of IL‐18 ± IL‐12 on MNCs IFN‐γ protein production and ex vivo expansion and activation of CB with IL‐12 + IL‐2 + anti‐CD3 ± IL‐18. Basal and activated levels of IL‐18 were significantly higher in PB versus CB MNCs (P 
ISSN:0007-1048
1365-2141
DOI:10.1111/j.1365-2141.2005.05592.x