Hormones and nutrients regulate acetyl-CoA carboxylase promoter I in rat primary hepatocytes

This study investigated the regulation of acetyl-CoA carboxylase (ACC) promoter activity by hormones and nutrients. Genomic clones including promoter I (PI) of the ACC gene were isolated and sequenced. ACC PI fragments (-1.049/ + 100 or -220/+21 bp) were subcloned into the pGL3-Basic vector that includes luciferase as a reporter gene. The ACC PI/luciferase chimeric plasmids were transfected into primary rat hepatocytes using lipofectin. Insulin treatment increased the activity of -1,049/4-100 and -22O/+21 ACC PI by 3.0- and 3,5-fold, respectively, compared to the control. The activity of both constructs was also increased by dexamethasone (Dex) and triiodothyronine (T3). with the greatest effects seen with all three hormones present. With -1.049/+100 or -220/+21 ACC PI, the addition of glucose increased luciferase activity compared to glucose-free control (p