NeuN expression correlates with reduced mitotic index of neoplastic cells in central neurocytomas

In the developing brain, neuronal differentiation is associated with permanent exit from the mitotic cycle. This raises the possibility that neuronal differentiation may suppress proliferative activity, even in neoplastic cells. As a first step towards understanding the relation between neuronal differentiation and mitotic cycling in brain tumours, we studied the expression of NeuN (a neuronal marker) and Ki‐67 (a mitotic marker) by double‐labelling immuno‐fluorescence in 16 brain tumours with neuronal differentiation. The tumours included a series of 11 central neurocytomas, and five single cases of other tumour types. In the central neurocytomas, NeuN+ cells had a 15‐fold lower Ki‐67 labelling index, on average, than did NeuN– cells (P