Early cellular changes of human mesenchymal stem cells and their interaction with other cells

Cell‐to–cell interactions between human mesenchymal stem cells and potential adjacent cells such as endothelial cells, dermal fibroblasts, and epidermal keratinocytes was investigated. A modified dual Boyden chamber assay using 8‐µm pores revealed a more powerful chemotactic cell migration of human mesenchymal stem cells toward human epidermal keratinocytes than other cells, such as umbilical artery endothelial cells and dermal fibroblasts, during 16 hours of incubation (336.2 ± 52.33, 36.0 ± 11.20, and 62.7 ± 18.16, cells/field, respectively, p