Rapid identification of bacteria by real-time amplification and sequencing of the 16S rRNA gene

To allow rapid identification of bacteria in pure cultures and blood culture bottles, an assay was developed which is based on real-time amplification and sequencing of bacterial 16 S rRNA genes. In principle, this assay allows identification of bacteria from pure cultures within 6.5 h, and from blo...

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Veröffentlicht in:	Journal of microbiological methods 2006-07, Vol.66 (1), p.156-164
Hauptverfasser:	Vliegen, Inge, Jacobs, Jan A., Beuken, Erik, Bruggeman, Cathrien A., Vink, Cornelis
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria, Aerobic - genetics Bacteria, Aerobic - isolation & purification Bacteria, Anaerobic - genetics Bacteria, Anaerobic - isolation & purification Bacteriology Biological and medical sciences DNA, Bacterial - chemistry DNA, Bacterial - genetics Escherichia coli - genetics Escherichia coli - isolation & purification Fluorescent Dyes - chemistry Fundamental and applied biological sciences. Psychology Genetics Microbiology Polymerase Chain Reaction - methods RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - genetics Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification
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Beschreibung
Zusammenfassung:	To allow rapid identification of bacteria in pure cultures and blood culture bottles, an assay was developed which is based on real-time amplification and sequencing of bacterial 16 S rRNA genes. In principle, this assay allows identification of bacteria from pure cultures within 6.5 h, and from blood cultures within approximately 7 h.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2005.11.005