Detection of Human Metapneumovirus From Children With Acute Otitis Media

Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positi...

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Veröffentlicht in:The Pediatric infectious disease journal 2005-07, Vol.24 (7), p.655-657
Hauptverfasser: Suzuki, Akira, Watanabe, Oshi, Okamoto, Michiko, Endo, Hiroko, Yano, Hisakazu, Suetake, Mitsuko, Nishimura, Hidekazu
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container_end_page 657
container_issue 7
container_start_page 655
container_title The Pediatric infectious disease journal
container_volume 24
creator Suzuki, Akira
Watanabe, Oshi
Okamoto, Michiko
Endo, Hiroko
Yano, Hisakazu
Suetake, Mitsuko
Nishimura, Hidekazu
description Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positive by this assay.
doi_str_mv 10.1097/01.inf.0000168755.01196.49
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subjects Acute Disease
Animals
Bacterial diseases
Bacterial Infections - complications
Bacterial Infections - microbiology
Biological and medical sciences
Cell Line
Child
Child, Preschool
Ear, Middle
Ent and stomatologic bacterial diseases
Female
Human bacterial diseases
Humans
Infant
Infectious diseases
Male
Medical sciences
Metapneumovirus - genetics
Metapneumovirus - isolation & purification
Otitis Media - microbiology
Otitis Media - virology
Paramyxoviridae Infections - complications
Paramyxoviridae Infections - virology
Polymerase Chain Reaction
Respiratory Tract Infections - complications
Respiratory Tract Infections - virology
Virus Diseases - complications
Virus Diseases - virology
title Detection of Human Metapneumovirus From Children With Acute Otitis Media
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