Detection of Human Metapneumovirus From Children With Acute Otitis Media

Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positi...

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Veröffentlicht in:	The Pediatric infectious disease journal 2005-07, Vol.24 (7), p.655-657
Hauptverfasser:	Suzuki, Akira, Watanabe, Oshi, Okamoto, Michiko, Endo, Hiroko, Yano, Hisakazu, Suetake, Mitsuko, Nishimura, Hidekazu
Format:	Artikel
Sprache:	eng
Schlagworte:	Acute Disease Animals Bacterial diseases Bacterial Infections - complications Bacterial Infections - microbiology Biological and medical sciences Cell Line Child Child, Preschool Ear, Middle Ent and stomatologic bacterial diseases Female Human bacterial diseases Humans Infant Infectious diseases Male Medical sciences Metapneumovirus - genetics Metapneumovirus - isolation & purification Otitis Media - microbiology Otitis Media - virology Paramyxoviridae Infections - complications Paramyxoviridae Infections - virology Polymerase Chain Reaction Respiratory Tract Infections - complications Respiratory Tract Infections - virology Virus Diseases - complications Virus Diseases - virology
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Beschreibung
Zusammenfassung:	Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positive by this assay.
ISSN:	0891-3668 1532-0987
DOI:	10.1097/01.inf.0000168755.01196.49