Laccase Production Using Pleurotus ostreatus 1804 Immobilized on PUF Cubes in Batch and Packed Bed Reactors: Influence of Culture Conditions

The feasibility of laccase production by immobilization of Pleurotus ostreatus 1804 on polyurethane foam (PUF) cubes with respect to media composition was studied in both batch and reactor systems. Enhanced laccase yield was evidenced due to immobilization. A relatively high maximum laccase activity...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The journal of microbiology 2005-06, Vol.43 (3), p.301-307
Hauptverfasser:	Prasad, K. Krishna (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India), Mohan, S. Venkata (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India), Bhaskar, Y. Vijaya (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India), Ramanaiah, S. V. (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India), Babu, V. Lalit (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India), Pati, B. R. (Vidyasagar University, Midnapore, India), Sarma, P.N. (Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India), E-mail: kousik@ins.iictnet.com
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioreactors Biotechnology - methods Cells, Immobilized culture condit conditions Culture Media - chemistry Hydrogen-Ion Concentration IMMOBILISATION IMMOBILIZATION INMOVILIZACION LACASA LACCASE Laccase - biosynthesis packed bed reactor Pleurotus - enzymology Pleurotus - growth & development Pleurotus ostreatus 1804 Polyurethanes
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The feasibility of laccase production by immobilization of Pleurotus ostreatus 1804 on polyurethane foam (PUF) cubes with respect to media composition was studied in both batch and reactor systems. Enhanced laccase yield was evidenced due to immobilization. A relatively high maximum laccase activity of 312.6 U was observed with immobilized mycelia in shake flasks compared to the maximum laccase activity of free mycelia (272.2 U). It is evident from this study that the culture conditions studied, i.e. biomass level, pH, substrate concentration, yeast extract concentration, Cu²+ concentration, and alcohol nature, showed significant influence on the laccase yield.
ISSN:	1225-8873 1976-3794