Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells

We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB...

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Veröffentlicht in:	FEBS letters 2005-07, Vol.579 (17), p.3473-3480
Hauptverfasser:	Islam, Mohammed Omedul, Kanemura, Yonehiro, Tajria, Jesmin, Mori, Hideki, Kobayashi, Satoshi, Shofuda, Tomoko, Miyake, Jun, Hara, Masayuki, Yamasaki, Mami, Okano, Hideyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	ABC transporter ABC transporters ABCB1 adenosinetriphosphatase antibodies ATP-binding cassette transporter ATP-Binding Cassette, Sub-Family B, Member 1 - analysis ATP-Binding Cassette, Sub-Family B, Member 1 - genetics ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Cell Differentiation cell membranes Cell surface marker Down-Regulation fractionation gene expression hNSPCs human neural stem/progenitor cells Humans immunocytochemistry Intermediate Filament Proteins - genetics Intermediate Filament Proteins - metabolism MDR microsomes multidrug resistance Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Nestin Neural stem cell Neurons - chemistry Neurons - metabolism P-glycoprotein P-gp Phenotype reverse transcriptase polymerase chain reaction Rh123 rhodamine 123 rhodamines RNA, Messenger - analysis RNA, Messenger - metabolism side-population Stem Cells - chemistry Stem Cells - metabolism
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Zusammenfassung:	We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs’ rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2005.05.019