Usefulness of CD45 density in the diagnosis of B-cell chronic lymphoproliferative disorders
Background: Although many B-cell chronic lymphoproliferative disorders (BCLPDs) including B-cell chronic lymphocytic leukemia (B-CLL) have characteristic clinical and biological features, the overlapping morphologic and immunophenotypic profiles of various BCLPDs, is still the main problem. Aim: Our...
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Veröffentlicht in: | Indian journal of medical sciences 2005-05, Vol.59 (5), p.187-194 |
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Sprache: | eng |
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Zusammenfassung: | Background: Although many B-cell chronic lymphoproliferative disorders
(BCLPDs) including B-cell chronic lymphocytic leukemia (B-CLL) have
characteristic clinical and biological features, the overlapping
morphologic and immunophenotypic profiles of various BCLPDs, is still
the main problem. Aim: Our aim was to evaluate the usefulness of CD45
expression in the immunological classification of BCLPDs. Setting and
design: A prospective study was set in a university hospital to
investigate the CD45 intensity, particularly in B-CLL. Materials and
Methods: The expression of CD45 in 37 patients with BCLPD including
typical B-CLL (Group I), atypical B-CLL and CLL/PLL (II), and hairy
cell leukemia (HCL), B-prolymphocytic leukemia (B-PLL), and B-non
Hodgkin′s lymphoma (B-NHL) as non-CLL BCLPDs (III) and in eight
healthy age matched controls (IV) was quantitatively compared by flow
cytometric CD45/RALS gating strategy. Statistical analysis: The mean,
median, and peak channel scores of CD45 obtained for the four groups
were compared using one-way analysis of variance test. A P value <
0.05 was to be considered statistically significant. Results: Lower
CD45 density is associated highly with typical CLL and differences
between typical CLL and other groups were significant (P>0.001,
0.001, and 0.001). Non-CLL cases had significantly brighter CD45
expression than atypical CLL (P=0.014). No differences were found
between normal lymphocytes and non-CLL BCLPD cases. Conclusions: CD45
is a useful marker, to discriminate the typical CLL from the non-CLL
BCLPD and from atypical CLL. |
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ISSN: | 0019-5359 1998-3654 |
DOI: | 10.4103/0019-5359.16254 |