Enhanced myocardial cathepsin B expression in patients with dilated cardiomyopathy
Abstract Objective Cathepsin B is a prominent lysosomal protease and is involved in apoptosis as well as degradation of myofibrillar proteins in myocardial infarction. The aim of this study was to investigate myocardial cathepsin B expression in failing and non-failing human hearts. Methods: Tissue...
Gespeichert in:
| Veröffentlicht in: | European journal of heart failure 2006-05, Vol.8 (3), p.284-289 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 289 |
|---|---|
| container_issue | 3 |
| container_start_page | 284 |
| container_title | European journal of heart failure |
| container_volume | 8 |
| creator | Ge, Junbo Zhao, Gang Chen, Ruizhen Li, Shuangjie Wang, Shijun Zhang, Xingang Zhuang, Yamin Du, Jiuzhong Yu, Xiaohua Li, Gaoping Yang, Yingzhen |
| description | Abstract
Objective
Cathepsin B is a prominent lysosomal protease and is involved in apoptosis as well as degradation of myofibrillar proteins in myocardial infarction. The aim of this study was to investigate myocardial cathepsin B expression in failing and non-failing human hearts.
Methods:
Tissue samples were taken from transplanted left ventricles from 20 patients with dilated cardiomyopathy and 5 non-failing donor hearts that could not be transplanted for technical reasons. Myocardial cathepsin B expression was determined by immunohistochemistry, the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Apoptosis was assessed by TUNEL staining.
Results:
Positive cathepsin B staining was found in failing and non-failing hearts. The expression of cathepsin B at mRNA and protein levels was significantly higher in failing hearts compared with non-failing hearts. Correlation analysis revealed that cathepsin B at mRNA and protein levels negatively correlated with EF (r=0.66, p=0.002 and r=0.492, p=0.028, respectively) in patients with heart failure. The apoptotic index was 0.015±0.006 in failing hearts and 0.002±0.001 in non-failing hearts (p |
| doi_str_mv | 10.1016/j.ejheart.2005.09.004 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67968403</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1016/j.ejheart.2005.09.004</oup_id><sourcerecordid>67968403</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4924-dc4db67d78979459aa72f0ae63b6e1a09accdb7d548fc4e3a91d70a43b071bd93</originalsourceid><addsrcrecordid>eNqNkM1u1DAURi0EoqXwCKCs2CW9jp3YXtJq2lKVIhA_S-vGvqN4yCRpnFE7b49LRiB2XdmWv_Nd-zD2lkPBgdenm4I2LeE0FyVAVYApAOQzdsy1MjloKZ-nvdA6N1qWR-xVjBsArgDKl-yI11KDKatj9nXVt9g78tl2PzicfMAuczi3NMbQZ2cZPYwTxRiGPkvnEedA_Ryz-zC3mQ8dzgn9ww2pIF23-9fsxRq7SG8O6wn7frH6dn6V33y-_Hj-4SZ30pQy9076plZeaaOMrAyiKteAVIumJo5g0DnfKF9JvXaSBBruFaAUDSjeeCNO2Puld5yGux3F2W5DdNR12NOwi7ZWptYSRApWS9BNQ4wTre04hS1Oe8vBPsq0G3uQaR9lWjA2yUzcu8OAXbMl_4862EuBsyVwHzraP63Vrq6vLv6fAkvJsBuf_LB8QUKc6eEvhNOv9GmhKvvz9tLyqhZfftyW9pP4DRnqp18</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67968403</pqid></control><display><type>article</type><title>Enhanced myocardial cathepsin B expression in patients with dilated cardiomyopathy</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><source>Wiley Open Access</source><source>EZB Electronic Journals Library</source><creator>Ge, Junbo ; Zhao, Gang ; Chen, Ruizhen ; Li, Shuangjie ; Wang, Shijun ; Zhang, Xingang ; Zhuang, Yamin ; Du, Jiuzhong ; Yu, Xiaohua ; Li, Gaoping ; Yang, Yingzhen</creator><creatorcontrib>Ge, Junbo ; Zhao, Gang ; Chen, Ruizhen ; Li, Shuangjie ; Wang, Shijun ; Zhang, Xingang ; Zhuang, Yamin ; Du, Jiuzhong ; Yu, Xiaohua ; Li, Gaoping ; Yang, Yingzhen</creatorcontrib><description>Abstract
Objective
Cathepsin B is a prominent lysosomal protease and is involved in apoptosis as well as degradation of myofibrillar proteins in myocardial infarction. The aim of this study was to investigate myocardial cathepsin B expression in failing and non-failing human hearts.
Methods:
Tissue samples were taken from transplanted left ventricles from 20 patients with dilated cardiomyopathy and 5 non-failing donor hearts that could not be transplanted for technical reasons. Myocardial cathepsin B expression was determined by immunohistochemistry, the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Apoptosis was assessed by TUNEL staining.
Results:
Positive cathepsin B staining was found in failing and non-failing hearts. The expression of cathepsin B at mRNA and protein levels was significantly higher in failing hearts compared with non-failing hearts. Correlation analysis revealed that cathepsin B at mRNA and protein levels negatively correlated with EF (r=0.66, p=0.002 and r=0.492, p=0.028, respectively) in patients with heart failure. The apoptotic index was 0.015±0.006 in failing hearts and 0.002±0.001 in non-failing hearts (p<0.01).
Conclusion:
Increased myocardial expression of cathepsin B was found in patients with heart failure suggesting that cathepsin B might play a role in the genesis and development of heart failure.</description><identifier>ISSN: 1388-9842</identifier><identifier>EISSN: 1879-0844</identifier><identifier>DOI: 10.1016/j.ejheart.2005.09.004</identifier><identifier>PMID: 16480925</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; Apoptosis ; Cardiomyopathy, Dilated - enzymology ; cathepsin B ; Cathepsin B - analysis ; Cathepsin B - genetics ; Child ; dilated cardiomyopathy ; Female ; heart failure ; Humans ; Immunohistochemistry ; In Situ Nick-End Labeling ; Male ; Middle Aged ; Myocardium - enzymology</subject><ispartof>European journal of heart failure, 2006-05, Vol.8 (3), p.284-289</ispartof><rights>2005 European Society of Cardiology 2005</rights><rights>Published on behalf of the European Society of Cardiology. All rights reserved. © 2006 the Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4924-dc4db67d78979459aa72f0ae63b6e1a09accdb7d548fc4e3a91d70a43b071bd93</citedby><cites>FETCH-LOGICAL-c4924-dc4db67d78979459aa72f0ae63b6e1a09accdb7d548fc4e3a91d70a43b071bd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.ejheart.2005.09.004$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.ejheart.2005.09.004$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16480925$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ge, Junbo</creatorcontrib><creatorcontrib>Zhao, Gang</creatorcontrib><creatorcontrib>Chen, Ruizhen</creatorcontrib><creatorcontrib>Li, Shuangjie</creatorcontrib><creatorcontrib>Wang, Shijun</creatorcontrib><creatorcontrib>Zhang, Xingang</creatorcontrib><creatorcontrib>Zhuang, Yamin</creatorcontrib><creatorcontrib>Du, Jiuzhong</creatorcontrib><creatorcontrib>Yu, Xiaohua</creatorcontrib><creatorcontrib>Li, Gaoping</creatorcontrib><creatorcontrib>Yang, Yingzhen</creatorcontrib><title>Enhanced myocardial cathepsin B expression in patients with dilated cardiomyopathy</title><title>European journal of heart failure</title><addtitle>European Journal of Heart Failure</addtitle><description>Abstract
Objective
Cathepsin B is a prominent lysosomal protease and is involved in apoptosis as well as degradation of myofibrillar proteins in myocardial infarction. The aim of this study was to investigate myocardial cathepsin B expression in failing and non-failing human hearts.
Methods:
Tissue samples were taken from transplanted left ventricles from 20 patients with dilated cardiomyopathy and 5 non-failing donor hearts that could not be transplanted for technical reasons. Myocardial cathepsin B expression was determined by immunohistochemistry, the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Apoptosis was assessed by TUNEL staining.
Results:
Positive cathepsin B staining was found in failing and non-failing hearts. The expression of cathepsin B at mRNA and protein levels was significantly higher in failing hearts compared with non-failing hearts. Correlation analysis revealed that cathepsin B at mRNA and protein levels negatively correlated with EF (r=0.66, p=0.002 and r=0.492, p=0.028, respectively) in patients with heart failure. The apoptotic index was 0.015±0.006 in failing hearts and 0.002±0.001 in non-failing hearts (p<0.01).
Conclusion:
Increased myocardial expression of cathepsin B was found in patients with heart failure suggesting that cathepsin B might play a role in the genesis and development of heart failure.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Apoptosis</subject><subject>Cardiomyopathy, Dilated - enzymology</subject><subject>cathepsin B</subject><subject>Cathepsin B - analysis</subject><subject>Cathepsin B - genetics</subject><subject>Child</subject><subject>dilated cardiomyopathy</subject><subject>Female</subject><subject>heart failure</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myocardium - enzymology</subject><issn>1388-9842</issn><issn>1879-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1u1DAURi0EoqXwCKCs2CW9jp3YXtJq2lKVIhA_S-vGvqN4yCRpnFE7b49LRiB2XdmWv_Nd-zD2lkPBgdenm4I2LeE0FyVAVYApAOQzdsy1MjloKZ-nvdA6N1qWR-xVjBsArgDKl-yI11KDKatj9nXVt9g78tl2PzicfMAuczi3NMbQZ2cZPYwTxRiGPkvnEedA_Ryz-zC3mQ8dzgn9ww2pIF23-9fsxRq7SG8O6wn7frH6dn6V33y-_Hj-4SZ30pQy9076plZeaaOMrAyiKteAVIumJo5g0DnfKF9JvXaSBBruFaAUDSjeeCNO2Puld5yGux3F2W5DdNR12NOwi7ZWptYSRApWS9BNQ4wTre04hS1Oe8vBPsq0G3uQaR9lWjA2yUzcu8OAXbMl_4862EuBsyVwHzraP63Vrq6vLv6fAkvJsBuf_LB8QUKc6eEvhNOv9GmhKvvz9tLyqhZfftyW9pP4DRnqp18</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Ge, Junbo</creator><creator>Zhao, Gang</creator><creator>Chen, Ruizhen</creator><creator>Li, Shuangjie</creator><creator>Wang, Shijun</creator><creator>Zhang, Xingang</creator><creator>Zhuang, Yamin</creator><creator>Du, Jiuzhong</creator><creator>Yu, Xiaohua</creator><creator>Li, Gaoping</creator><creator>Yang, Yingzhen</creator><general>Blackwell Publishing Ltd</general><general>Elsevier</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200605</creationdate><title>Enhanced myocardial cathepsin B expression in patients with dilated cardiomyopathy</title><author>Ge, Junbo ; Zhao, Gang ; Chen, Ruizhen ; Li, Shuangjie ; Wang, Shijun ; Zhang, Xingang ; Zhuang, Yamin ; Du, Jiuzhong ; Yu, Xiaohua ; Li, Gaoping ; Yang, Yingzhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4924-dc4db67d78979459aa72f0ae63b6e1a09accdb7d548fc4e3a91d70a43b071bd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Apoptosis</topic><topic>Cardiomyopathy, Dilated - enzymology</topic><topic>cathepsin B</topic><topic>Cathepsin B - analysis</topic><topic>Cathepsin B - genetics</topic><topic>Child</topic><topic>dilated cardiomyopathy</topic><topic>Female</topic><topic>heart failure</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Myocardium - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ge, Junbo</creatorcontrib><creatorcontrib>Zhao, Gang</creatorcontrib><creatorcontrib>Chen, Ruizhen</creatorcontrib><creatorcontrib>Li, Shuangjie</creatorcontrib><creatorcontrib>Wang, Shijun</creatorcontrib><creatorcontrib>Zhang, Xingang</creatorcontrib><creatorcontrib>Zhuang, Yamin</creatorcontrib><creatorcontrib>Du, Jiuzhong</creatorcontrib><creatorcontrib>Yu, Xiaohua</creatorcontrib><creatorcontrib>Li, Gaoping</creatorcontrib><creatorcontrib>Yang, Yingzhen</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of heart failure</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ge, Junbo</au><au>Zhao, Gang</au><au>Chen, Ruizhen</au><au>Li, Shuangjie</au><au>Wang, Shijun</au><au>Zhang, Xingang</au><au>Zhuang, Yamin</au><au>Du, Jiuzhong</au><au>Yu, Xiaohua</au><au>Li, Gaoping</au><au>Yang, Yingzhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced myocardial cathepsin B expression in patients with dilated cardiomyopathy</atitle><jtitle>European journal of heart failure</jtitle><addtitle>European Journal of Heart Failure</addtitle><date>2006-05</date><risdate>2006</risdate><volume>8</volume><issue>3</issue><spage>284</spage><epage>289</epage><pages>284-289</pages><issn>1388-9842</issn><eissn>1879-0844</eissn><abstract>Abstract
Objective
Cathepsin B is a prominent lysosomal protease and is involved in apoptosis as well as degradation of myofibrillar proteins in myocardial infarction. The aim of this study was to investigate myocardial cathepsin B expression in failing and non-failing human hearts.
Methods:
Tissue samples were taken from transplanted left ventricles from 20 patients with dilated cardiomyopathy and 5 non-failing donor hearts that could not be transplanted for technical reasons. Myocardial cathepsin B expression was determined by immunohistochemistry, the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Apoptosis was assessed by TUNEL staining.
Results:
Positive cathepsin B staining was found in failing and non-failing hearts. The expression of cathepsin B at mRNA and protein levels was significantly higher in failing hearts compared with non-failing hearts. Correlation analysis revealed that cathepsin B at mRNA and protein levels negatively correlated with EF (r=0.66, p=0.002 and r=0.492, p=0.028, respectively) in patients with heart failure. The apoptotic index was 0.015±0.006 in failing hearts and 0.002±0.001 in non-failing hearts (p<0.01).
Conclusion:
Increased myocardial expression of cathepsin B was found in patients with heart failure suggesting that cathepsin B might play a role in the genesis and development of heart failure.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>16480925</pmid><doi>10.1016/j.ejheart.2005.09.004</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1388-9842 |
| ispartof | European journal of heart failure, 2006-05, Vol.8 (3), p.284-289 |
| issn | 1388-9842 1879-0844 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67968403 |
| source | MEDLINE; Wiley Online Library All Journals; Wiley Open Access; EZB Electronic Journals Library |
| subjects | Adolescent Adult Apoptosis Cardiomyopathy, Dilated - enzymology cathepsin B Cathepsin B - analysis Cathepsin B - genetics Child dilated cardiomyopathy Female heart failure Humans Immunohistochemistry In Situ Nick-End Labeling Male Middle Aged Myocardium - enzymology |
| title | Enhanced myocardial cathepsin B expression in patients with dilated cardiomyopathy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T11%3A14%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhanced%20myocardial%20cathepsin%20B%20expression%20in%20patients%20with%20dilated%20cardiomyopathy&rft.jtitle=European%20journal%20of%20heart%20failure&rft.au=Ge,%20Junbo&rft.date=2006-05&rft.volume=8&rft.issue=3&rft.spage=284&rft.epage=289&rft.pages=284-289&rft.issn=1388-9842&rft.eissn=1879-0844&rft_id=info:doi/10.1016/j.ejheart.2005.09.004&rft_dat=%3Cproquest_cross%3E67968403%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67968403&rft_id=info:pmid/16480925&rft_oup_id=10.1016/j.ejheart.2005.09.004&rfr_iscdi=true |