Enhanced myocardial cathepsin B expression in patients with dilated cardiomyopathy

Abstract Objective Cathepsin B is a prominent lysosomal protease and is involved in apoptosis as well as degradation of myofibrillar proteins in myocardial infarction. The aim of this study was to investigate myocardial cathepsin B expression in failing and non-failing human hearts. Methods: Tissue...

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Veröffentlicht in:European journal of heart failure 2006-05, Vol.8 (3), p.284-289
Hauptverfasser: Ge, Junbo, Zhao, Gang, Chen, Ruizhen, Li, Shuangjie, Wang, Shijun, Zhang, Xingang, Zhuang, Yamin, Du, Jiuzhong, Yu, Xiaohua, Li, Gaoping, Yang, Yingzhen
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Sprache:eng
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Zusammenfassung:Abstract Objective Cathepsin B is a prominent lysosomal protease and is involved in apoptosis as well as degradation of myofibrillar proteins in myocardial infarction. The aim of this study was to investigate myocardial cathepsin B expression in failing and non-failing human hearts. Methods: Tissue samples were taken from transplanted left ventricles from 20 patients with dilated cardiomyopathy and 5 non-failing donor hearts that could not be transplanted for technical reasons. Myocardial cathepsin B expression was determined by immunohistochemistry, the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Apoptosis was assessed by TUNEL staining. Results: Positive cathepsin B staining was found in failing and non-failing hearts. The expression of cathepsin B at mRNA and protein levels was significantly higher in failing hearts compared with non-failing hearts. Correlation analysis revealed that cathepsin B at mRNA and protein levels negatively correlated with EF (r=0.66, p=0.002 and r=0.492, p=0.028, respectively) in patients with heart failure. The apoptotic index was 0.015±0.006 in failing hearts and 0.002±0.001 in non-failing hearts (p
ISSN:1388-9842
1879-0844
DOI:10.1016/j.ejheart.2005.09.004