Bisphenol-A induces cell cycle delay and alters centrosome and spindle microtubular organization in oocytes during meiosis

Bisphenol-A (BPA) is a widely used environmental estrogen-like chemical that has a weak estrogenic activity. This study aimed to test the potential inhibitory effects of BPA on meiotic cell cycle progression, centrosomes and spindle integrity in mouse cumulus–oocyte complexes (COCs). They were exposed to BPA (10–30 μM; 2.3–6.8 ppm) during meiosis-I and the formation of metaphase-II (M-II) spindle. Exposure to BPA during meiosis-I caused a dose-dependent retardation/inhibition of cell cycle progression; 74 and 61% of cells reached metaphase-I (M-I) in the presence of 10 and 30 μM BPA, respectively, (81% in controls, P