Functional analysis of immune response genes in Drosophila identifies JNK pathway as a regulator of antimicrobial peptide gene expression in S2 cells
The templates of innate immunity have ancient origins. Thus, such model animals as the fruit fly, Drosophila melanogaster, can be used to identify gene products that also play a key role in the innate immunity in mammals. We have used oligonucleotide microarrays to identify genes that are responsive...
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Veröffentlicht in: | Microbes and infection 2005-05, Vol.7 (5), p.811-819 |
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Sprache: | eng |
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Zusammenfassung: | The templates of innate immunity have ancient origins. Thus, such model animals as the fruit fly,
Drosophila melanogaster, can be used to identify gene products that also play a key role in the innate immunity in mammals. We have used oligonucleotide microarrays to identify genes that are responsive to Gram-negative bacteria in
Drosophila macrophage-like S2 cells. In total, 53 genes were induced by greater than threefold in response to
Escherichia coli. The induction of all these genes was peptidoglycan recognition protein LC (PGRP-LC) dependent. Twenty-two genes including 10 of the most strongly induced genes are also known to be up-regulated by septic injury in vivo. Importantly, we identified 31 genes that are not known to respond to bacterial challenge. We carried out targeted dsRNA treatments to assess the functional importance of these gene products for microbial recognition, phagocytosis and antimicrobial peptide release in
Drosophila S2 cells in vitro. RNAi targeting three of these genes,
CG7097,
CG15678 and
β-Tubulin
60D, caused altered antimicrobial peptide release in vitro. Our results indicate that the JNK pathway is essential for normal antimicrobial peptide release in
Drosophila in vitro. |
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ISSN: | 1286-4579 1769-714X |
DOI: | 10.1016/j.micinf.2005.03.014 |