Laser pressure catapulting followed by B actin gene identification in Japanese quail macrochromosomes and microchromosomes using teflon‐coated coverslip slides

Laser pressure catapulting followed by B actin gene identification in Japanese quail macrochromosomes and microchromosomes using teflon‐coated coverslip slides

Summary Laser microdissection of individual mammalian chromosomes (> 2 µm) has been achieved though the use of a microscope slide coated with a polyethylene naphthalate (PEN) membrane. Although these slides have proved sufficient for larger chromosomes, they are insufficient for small chromosomes...

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Veröffentlicht in:Journal of microscopy (Oxford) 2005-06, Vol.218 (3), p.219-224
Hauptverfasser: MCNALLY, L. R., HENK, W. G., COOPER, R. K.
Format: Artikel
Sprache:eng
Schlagworte:
Actins - genetics
Animals
Avian chromosomes
Base Sequence
Cell Fractionation - methods
Chromosomes
Consensus Sequence
Coturnix
Coturnix japonica
laser microdissection
laser pressure catapulting
Lasers
Microdissection - methods
Microscopy - instrumentation
Microscopy - methods
Molecular Sequence Data
Polytetrafluoroethylene
Sequence Homology, Nucleic Acid
Surface Properties
Teflon‐coated coverslip slides
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Beschreibung
Zusammenfassung:Summary Laser microdissection of individual mammalian chromosomes (> 2 µm) has been achieved though the use of a microscope slide coated with a polyethylene naphthalate (PEN) membrane. Although these slides have proved sufficient for larger chromosomes, they are insufficient for small chromosomes (
ISSN:0022-2720
1365-2818
DOI:10.1111/j.1365-2818.2005.01479.x