Peptide ELISA for measuring antibodies to N-protein of porcine reproductive and respiratory syndrome virus

Indirect and competition ELISAs with synthetic peptides were used to characterize the epitopes of the N-protein of porcine reproductive and respiratory syndrome virus (PRRSV) that are recognized by a battery of monoclonal antibodies (mAbs) and by antibodies from infected pigs. Four linear epitopes recognized by mAbs have been identified in the most hydrophilic segment of the N-protein (AA25–57). Similarly, at least four linear epitopes in this segment are immunogenic in PRRSV-infected pigs, but only one corresponds to an epitope recognized by one of the mAbs (AA36–45). Antibody formation to these epitopes varied greatly between individual pigs. Most infected pigs generated antibodies that bound to both peptides and HerdChek plates, which are commonly used in the sero-diagnosis of PRRSV infections, but the time course of formation of peptide binding antibodies and antibodies that react with HerdChek plates differed greatly between pigs. This suggests that, although the peptide and HerdChek ELISAs may detect antibodies to some of the same epitopes, they also seem to detect antibodies to epitopes that are uniquely expressed by one and not the other. Some mAbs fail to bind to HerdChek ELISA plates and this is also the case for certain pig antibodies. Peptide ELISA results identified four herds in which most or all pigs possessed N-protein peptide binding antibodies, even though they were HerdChek ELISA sero-negative and exhibited no other signs of PRRSV infection. Thus PRRSV infections may be more widespread than presently realized involving strains that cause asymptomatic infections. The peptide ELISA is useful as an adjunct to the HerdChek ELISA or it could replace it since only two serum samples among 450 tested were HerdChek ELISA positive but peptide ELISA negative. The peptide ELISA is also considerably cheaper than the HerdChek ELISA, more flexible and can provide information on the epitope specificity of the reacting antibodies.