Macromolecular oxidation in anisotonic suspensions of mouse spleen cells

Macromolecular oxidative alterations have been analysed in vitro in anisotonic suspensions of mouse splenocytes. Both hypertonicity and hypotonicity induced the generation of thiobarbituric acid reactive species (TBARS) and carbonylation of the proteins, which took place along with cell death. Addit...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Cell biochemistry and function 2006-05, Vol.24 (3), p.201-207
Hauptverfasser:	Bilić, Ivan, Kovac, Zdenko
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals anisotonicity Cell Death - drug effects Hypertonic Solutions - pharmacology Hypotonic Solutions - pharmacology In Vitro Techniques lipid peroxidation Macromolecular Substances - analysis Mice Mice, Inbred CBA Osmotic Pressure - drug effects osmotic stress Oxidation-Reduction - drug effects oxidative stress Oxidative Stress - drug effects protein carbonylation Protein Carbonylation - drug effects Protein Carbonylation - physiology Spleen - cytology Spleen - drug effects Spleen - metabolism splenocytes TBARS Thiobarbituric Acid Reactive Substances - analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Macromolecular oxidative alterations have been analysed in vitro in anisotonic suspensions of mouse splenocytes. Both hypertonicity and hypotonicity induced the generation of thiobarbituric acid reactive species (TBARS) and carbonylation of the proteins, which took place along with cell death. Addition of antioxidants partially inhibited oxidative changes in isotonic and hypotonic suspensions. Anisotonic shock of mouse splenocytes proved to be an inducer of oxidative stress. The oxidative macromolecular alterations might contribute to pathogenesis of cell death caused by osmotic stress. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:	0263-6484 1099-0844
DOI:	10.1002/cbf.1207